Fig. 1.

Hippo signaling is required for eye development. (A,B) Wild-type eye imaginal disc and compound eye of adult fly are shown. (A) Eye imaginal disc is stained for the membrane-specific marker Discs large (Dlg, green) and the pan-neural marker Elav (red), which marks the retinal neuron-specific fate. (C,E,G) Eye discs in which bi-Gal4 drives misexpression of (C) full-length yki (bi>yki-GFP), and its hyper activated forms (E) yki^S168A (bi>yki^S168A) and (G) bi>yki^3SA (bi>yki^3SA). The resulting adult eyes are shown in D,F,H respectively. Hyperactivated Yki resulted in suppression of the eye fate on both dorsal and ventral margins of (C,E,G) the eye disc and (D,F,H) the adult eye. (C,E) GFP (blue) marks the bi-Gal4 driver domain in the eye disc. (I,J) Misexpression of yki^RNAi on DV margins (bi>dicer+yki^RNAi(N+C)) resulted in enlargement of the eye field at both dorsal and ventral margins of the (I) eye disc and in the (J) adult. The orientation of all imaginal discs is identical with posterior towards the left and dorsal upwards.