Fig. 6.

Activation of yki suppresses the eye fate by ectopically inducing Wg signaling. (A,A′) Panels show wild-type Wg (green) expression in the third instar eye imaginal disc. Wg (green) is expressed antero-laterally on dorsal and ventral eye margins of the eye imaginal disc. (B,C) Misexpression of yki^3SA, under (B-B″) bi-Gal4 (bi>yki^3SA) at DV margins or in ‘FLP-out’ random clones (marked by GFP, blue) under (C-C″) hsFLP>yki^3SA in the eye disc, results in suppression of eye fate (marked by Elav, red) and ectopic induction of Wg expression (green, marked by white arrows in B,B′,C′). Blocking Wg signaling in bi>yki eye disc by misexpression of antagonists of Wg signaling such as (D,E) dominant-negative dTCF^DN (bi>yki+dTCF^DN5) and (F,G) Shaggy (Sgg) (bi>yki+Sgg^S9A), resulted in the rescue of eye suppression by yki (bi>yki) in the (D,F) eye disc and (E,G) the adult eye. The orientation and magnification of imaginal discs and adult heads are identical in all panels.