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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Neuroendocrinology. 2019 Jun 19;110(1-2):105–118. doi: 10.1159/000501560

Fig. 4. E2 replacement rescues insulin response in NPY/AgRP neurons from DIO, ovariectomized females.

Fig. 4.

A, insulin (20 nM) induced an outward current in voltage clamp in NPY/AgRP neurons from control diet-fed, ovariectomized female mice. The insulin responses were antagonized by KATP channel blocker tolbutamide. Vhold = −60 mV. B, in DIO, ovariectomized females, the insulin response was abrogated in NPY/AgRP neurons. C, however, the insulin responses in voltage clamp were rescued in DIO, ovariectomized females with EB treatment. D, bar graphs summarizing the insulin-induced currents in normal diet and DIO, ovariectomized and E2-treated female mice. Data points represent the mean ± SEM (control diet, OVX females: 14.1 ± 2.7 pA, n = 7; DIO, OVX females: 1.4 ± 0.9 pA, n = 9; DIO, EB-treated, OVX females: 9.6 ± 2.2 pA, n = 12; one-way ANOVA, effect of treatment, F (2, 25) = 8.531, p = 0.0015; Newman-Keuls’ multiple-comparison post-hoc test. ** p < 0.01; ns, no significant difference). Cell numbers are indicated.