Table 6.
The effects of differing intestinal bacterial communities on tight junction, mucin-2 and immune-related genes’ expressions in broiler chickens on the 21st day.
| Treatment | claudin-1 | occludin | mucin-2 | IFN-γ | TLR2 | TLR4 | NF-κB | MyD88 | |
|---|---|---|---|---|---|---|---|---|---|
| CON | 1.04 | 1.02 | 1.02 a | 1.04 a | 1.03 ab | 1.01 | 1.01 | 1.02 | |
| Anti G+ | 1.12 | 0.93 | 1.20 a | 1.37 ab | 0.88 a | 1.24 | 1.33 | 1.27 | |
| Anti G− | 0.89 | 0.94 | 1.28 a | 1.26 a | 0.95 a | 1.1 | 1.09 | 1.19 | |
| CONS | 0.71 | 0.69 | 1.01 a | 1.74 b | 1.08 ab | 1.32 | 0.98 | 0.96 | |
| Anti G+S | 0.52 | 0.78 | 1.74 b | 1.80 b | 1.32 a | 1.54 | 1.15 | 1.27 | |
| Anti G−S | 0.64 | 0.93 | 1.40 ab | 1.32 ab | 1.15 ab | 1.5 | 1.1 | 1.06 | |
| SEM | 0.05 | 0.04 | 0.06 | 0.06 | 0.04 | 0.05 | 0.03 | 0.03 | |
| Main effect | |||||||||
| Bacterial community | CON | 0.88 | 0.85 | 1.01 a | 1.39 ab | 1.06 | 1.17 | 1.00 a | 0.99 a |
| Anti G+ | 0.82 | 0.85 | 1.47 b | 1.59 a | 1.1 | 1.39 | 1.24 b | 1.27 b | |
| Anti G− | 0.77 | 0.93 | 1.34 b | 1.29 b | 1.05 | 1.3 | 1.11 ab | 1.12 ab | |
| S. typhimurium | − | 1.02 | 0.96 | 1.16 | 1.22 | 0.95 | 1.11 | 1.14 | 1.15 |
| + | 0.63 | 0.81 | 1.35 | 1.62 | 1.18 | 1.46 | 1.08 | 1.10 | |
| p value | |||||||||
| Feed | 0.448 | 0.481 | <0.001 | 0.026 | 0.742 | 0.054 | 0.009 | <0.001 | |
| S. typhimurium | <0.001 | 0.017 | 0.008 | <0.001 | 0.001 | <0.001 | 0.299 | 0.256 | |
| Bacterial community * S. typhimurium | 0.137 | 0.135 | 0.018 | 0.012 | 0.045 | 0.841 | 0.426 | 0.568 | |
Means in the same row with different superscript letters differ significantly (p < 0.05). Bacterial community * S. typhimurium: the interaction effect between the bacterial community and S. typhimurium.