Table 3.
HPLC method validation results of unconjugated bile acids (u-BA) in mice plasma and eyes (n = 3) 1.
| Bile Acid (Peak No.) | Plasma | Eyes | ||||
|---|---|---|---|---|---|---|
| Conc. (μM) | Precision (%) | Accuracy (%) | Conc. (μM) | Precision (%) | Accuracy (%) | |
| u-BA | 0.2 | 9.01 | 102 (10.82) | 0.2 | 36.77 | 104 (20.36) |
| 0.5 | 4.55 | 99 (4.57) | 0.5 | 7.24 | 99 (4.74) | |
| UDCA (3) | 1 | 8.58 | 96 (7.31) | 2 | 14.56 | 108 (19.22) |
| 10 | 4.26 | 106 (2.80) | 10 | 6.67 | 114 (2.77) | |
| 100 | 5.08 | 101 (1.37) | 100 | 2.70 | 99 (0.91) | |
| CA (6) | 0.2 | 7.19 | 96 (13.40) | 0.1 | 22.42 | 89 (13.90) |
| 0.5 | 4.89 | 102 (3.64) | 0.5 | 15.18 | 104 (12.17) | |
| 1 | 8.67 | 98 (7.43) | 1 | 4.62 | 99 (2.99) | |
| 10 | 5.32 | 103 (2.90) | 10 | 7.73 | 108 (4.22) | |
| 100 | 2.27 | 101 (1.20) | 100 | 3.14 | 99 (0.79) | |
| CDCA (11) | 0.2 | 23.81 | 101 (10.02) | 0.1 | 31.96 | 90 (6.72) |
| 0.5 | 5.66 | 101 (7.62) | 0.5 | 16.60 | 96 (7.28) | |
| 1 | 9.77 | 95 (7.66) | 1 | 7.46 | 101 (1.25) | |
| 10 | 5.39 | 103 (2.86) | 10 | 12.03 | 109 (8.10) | |
| 100 | 3.05 | 101 (1.25) | 100 | 4.93 | 99 (1.27) | |
| DCA (12) | 0.2 | 5.73 | 104 (10.53) | 0.1 | 27.87 | 102 (13.76) |
| 0.5 | 4.25 | 100 (5.18) | 0.5 | 19.08 | 96 (5.35) | |
| 1 | 8.22 | 96 (8.06) | 1 | 5.91 | 101 (1.07) | |
| 10 | 4.74 | 103 (3.07) | 10 | 11.26 | 111 (7.34) | |
| 100 | 4.28 | 101 (1.23) | 100 | 4.51 | 99 (1.01) | |
| LCA (16) | 0.2 | 17.75 | 99 (9.72) | 0.2 | 21.92 | 86 (8.31) |
| 0.5 | 12.08 | 102 (11.57) | 0.5 | 22.05 | 101 (12.31) | |
| 1 | 12.07 | 98 (12.86) | 1 | 3.35 | 100 (2.75) | |
| 10 | 4.66 | 102 (2.89) | 10 | 9.60 | 105 (6.72) | |
| 100 | 4.02 | 101 (1.17) | 100 | 4.81 | 100 (1.51) | |
1 RSD (residual deviation, %) values are displayed in the parenthesis for the averaged accuracy (%) values.