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. 2019 Oct 31;9(11):677. doi: 10.3390/biom9110677

Figure 7.

Figure 7

Dipterose-BSF induces the activation of NF-κB via the degradation of IκBα in macrophages. (A) RAW264.7 cells were treated with dipterose-BSF (100 ng/mL) or LPS (100 ng/mL) for 0, 15, 30, 45, or 60 min. The expression level of IκBα was detected using immunoblot analysis. α-tubulin served as a protein loading control. The same blot was cut and blotted using anti-mouse α-tubulin antibody. (B) RAW264.7 cells were incubated with dipterose-BSF (100 ng/mL) or LPS (100 ng/mL) for 0, 15, 30, 45, or 60 min. The expression level of NF-κB was monitored using immunoblot analysis. Lamin A/C was used as a protein loading control. The same blot was stripped and re-blotted using anti-lamin A/C antibody. Immunoblot images shown here are representative of two independent experiments. Data were expressed as means ± SEM of two samples. Quantitative analysis of protein level on the blot was calculated using ImageJ software (version 1.52a).