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. 2019 Oct 26;9(11):659. doi: 10.3390/biom9110659

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of polyphenols on Pseudomonas biofilm. (A) The amount of biofilm formed in the presence of polyphenols (100 µM) by Pseudomonas overexpressing the fap UK4 operon; (B) correlation between the amount of biofilm and endpoints level of ThT fluorescence for FapC protein incubated with and without polyphenols; (C) confocal microscopy images of pFap and wt incubated with and without 100 µM PGG and EGCG. The bacteria were stained with live/dead stain: Red and green denote dead and live bacteria, respectively, all images were taken with 63× oil objective, scale bars are 10 µm.

Effect of polyphenols on Pseudomonas biofilm. (A) The amount of biofilm formed in the presence of polyphenols (100 µM) by Pseudomonas overexpressing the fap UK4 operon; (B) correlation between the amount of biofilm and endpoints level of ThT fluorescence for FapC protein incubated with and without polyphenols; (C) confocal microscopy images of pFap and wt incubated with and without 100 µM PGG and EGCG. The bacteria were stained with live/dead stain: Red and green denote dead and live bacteria, respectively, all images were taken with 63× oil objective, scale bars are 10 µm.