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. 2019 Nov 29;50:274–289. doi: 10.1016/j.ebiom.2019.11.026

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 6 — Secretome analysis of C9 mutated astrocytes. a. Mass spectrometry analysis of control (Contr-L3, Contr-L9) and C9-mutated (C9-L5, C9-L8) astrocyte secretome. 3 biological replicates of media conditioned by each line were analyzed. Conditioned media was collected during toxicity experiments at 70–90 dFD of astrocytes. The results are presented by a volcano plot where each point represents an identified protein. The log2 difference intensity is plotted against the t-test p-value for each protein. The proteins with significantly (p < < 0.05) different levels between C9- and non-mutated secretome samples are in red. b. Proteins related to oxidative stress were significantly down-regulated in the C9-mutated secretome. (c–e) Down-regulation of SOD1, SOD2, and GSS in the C9-mutated secretome was validated by ELISA. 3 biological replicates of conditioned media from C9-mutated (C9-L5, C9-L8) and control (Contr-L3, Contr-L9) astrocytes were analyzed by ELISA assay. Data are presented as a range of values from 3 independent experiments.