Figure 4.
Mice Can Discriminate Two Distinct Artificial Activity Patterns in Auditory Cortex
(A) Sketch of the Go/NoGo discrimination task for optogenetic stimuli with sample trials showing licking signals for each stimulus.
(B) Sample cranial window superimposed with the location of two optogenetic stimuli in AC.
(C) Localization of the two optogenetic stimuli in the AC tonotopic map obtained from intrinsic imaging; same mouse as in (B).
(D) Population learning curve for discrimination of two optogenetic stimulations (340 ± 105 trials to reach 80% correct performance; n = 5 mice).
(E) Mean decision times for the three different discrimination tasks (optogenetic 424 ± 58 ms, n = 5 mice; FMvsPT, 366 ± 25 ms, n = 29 mice; PTvsPT, 153 ± 22 ms, n = 30 mice). PTvsPT was significantly different from the two other groups (p = 7.3 × 10−9 for FMvsPT and p = 1.1 × 10−3 for optogenetic; Wilcoxon rank-sum test), which were not different from each other (p = 0.2; Wilcoxon rank-sum test).
(F) Mean licking traces for S+ and S− stimuli during optogenetic (top), FMvsPT (middle), and PTvsPT (bottom) discrimination. Arrowhead, unspecific initial licks; gray shading, typical early response delay; green shading, typical late discrimination delay.