Figure 5.

Focal Optogenetic AC Activation Biases Decisions Only in the FMvsPT Task

(A) Intrinsic imaging response maps for three pure tones aligned and averaged across 10 mice. Bottom right: map of tonotopic gradients obtained by subtracting the 4- and 32-kHz response maps is shown. The approximate contours and direction of the main tonotopic gradients (primary auditory field A1, anterior auditory field AAF, and secondary auditory field A2) are superimposed.

(B) Schematic of the focal AC perturbation experiment: in occasional non-rewarded trials, the S− sound (4-kHz) was played synchronously with a local optogenetic stimulation in one out of 17 predefined locations aligned to the tonotopic map.

(C) Probability distribution of S− lick counts averaged over three random trials in one sample mouse. The superimposed red circles represent the mean lick counts over three trials for each optogenetic stimulus location. Open circles, non-significant responses; filled circles, lick count is larger than 95% of S− lick counts; filled circles with black contours, significant locations after Benjamini-Hochberg correction for multiple testing (p < 0.05).

(D) Map of normalized lick counts for optogenetic perturbations obtained by summing the estimated 2D neuronal population response profile for one light disk, multiplied by observed lick count and positioned at all optogenetic locations with a significant response.

(E) Mean lick counts for all optogenetic stimulus locations and all mice involved in the PTvsPT or FMvsPT tasks. Red filled circles correspond to significant locations (p < 0.05; corrected for multiple testing).

(F) Percentage of significant locations found in the PTvsPT and FMvsPT tasks across all mice. Data are represented as mean ± SEM. Dashed line, expected false discovery rate.

(G) Maps of normalized lick counts for significant optogenetic locations averaged across all mice performing the PTvsPT task. Black circles, centers of optogenetic stimuli.

(H) Same as (G) but for the FMvsPT task.