Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifier | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Kdrlox/lox | Haigh et al., 2003 | ||
| Genetic reagent (Mus musculus) | Nestin-cre | Tronche et al., 1999 | ||
| Genetic reagent (Mus musculus) | Vegfr2-GFP | Ema et al., 2006 | ||
| Genetic reagent (Mus musculus) | Efnb2lox/lox | Grunwald et al., 2004 | ||
| Antibody | rabbit anti-GFP polyclonal antibody | Invitrogen | Cat# A11122 RRID:AB_221569 |
1:200 IF |
| Antibody | chicken anti-GFP polyclonal antibody | Aves | Cat# GFP-1020 RRID:AB_10000240 |
1:200 IF |
| Antibody | rabbit anti-GFAP polyclonal antibody | Dako | Cat# Z0334 RRID:AB_10013382 |
1:100 IF |
| Antibody | mouse anti-NeuN monoclonal antibody | Millipore | Cat# MAB377 RRID:AB_2298772 |
1:50 IF |
| Antibody | rat anti-L1 monoclonal antibody | Millipore | Cat# MAB5272 RRID:AB_2133200 |
1:100 IF |
| Antibody | rabbit anti-Calretinin polyclonal antibody | Millipore | Cat# MAB5054 | 1:500 IF |
| Antibody | goat anti-Nrp1 polyclonal antibody | R and D | Cat# AF566 RRID:AB_355445 |
2 µg/ml for functional blocking |
| Antibody | goat anti-VEGFR2 polyclonal antibody |
R and D | Cat# AF644 RRID:AB_355500 |
1:15 IF |
| Antibody | rabbit anti-VEGFR2 monoclonal antibody | Cell Signaling | Cat# 2479 RRID:AB_2212507 |
1:1000 WB |
| Antibody | rabbit anti-(phospho)VEGFR2 (Y1175) | Cell Signaling | Cat# 2478 RRID:AB_331377 |
1:500 WB |
| Antibody | rabbit anti-(phospho)SFK (Y416) monoclonal antibody | Invitrogen | Cat# 44660G RRID:AB_2533714 |
1:200 IF |
| Antibody | mouse anti-beta-III-tubulin monoclonal antibody | Sigma | Cat# T5076 RRID:AB_532291 |
1:150 IF |
| Antibody | goat anti-VE-Cadherin polyclonal antibody | R and D | Cat# AF1002 RRID:AB_2077789 |
1:1000 WB |
| Antibody | donkey anti-rabbit Alexa488 polyclonal antibody | Jackson Immunoresearch | Cat# 711-545-152 RRID:AB_2313584 |
1:1000 IF |
| Antibody | donkey anti-rabbit Alexa568 polyclonal antibody | Life Technologies | Cat# A10042 RRID:AB_2534017 |
1:1000 IF |
| Antibody | donkey anti-goat Alexa568 polyclonal antibody | Molecular Probes | Cat# A11057 RRID:AB_142581 |
1:1000 IF |
| Antibody | goat anti-mouse Alexa568 polyclonal antibody | Invitrogen | Cat# A11031 RRID:AB_144696 |
1:1000 IF |
| Antibody | goat anti-rat Alexa568 polyclonal antibody | Invitrogen | Cat# A11077 RRID:AB_2534121 |
1:1000 IF |
| Antibody | goat anti-chicken Alexa488 polyclonal antibody | Molecular Probes | Cat# A11039 RRID:AB_142924 |
1:1000 IF |
| Antibody | donkey anti-goat HRP polyclonal antibody | Jackson Immunoresearch | Cat# 705-035-147 RRID:AB_2313587 |
1:1000 IF |
| Antibody | donkey anti-rabbit HRP polyclonal antibody | Jackson Immunoresearch | Cat# 711-035-152 RRID:AB_10015282 |
1:1000 IF |
| Antibody | donkey anti-mouse HRP polyclonal antibody | Jackson Immunoresearch | Cat# 715-035-150 RRID:AB_2340770 |
1:1000 IF |
| Chemical compound, drug | Phalloidin-TRITC | Sigma | Cat# P1951 RRID:AB_2315148 |
1:400 IF |
| Chemical compound, drug | dynasore | Selleckchem | Cat# S8047 | 80 µM for functional blocking |
| Chemical compound, drug | DiI lipophilic tracer dye | Molecular Probes | Cat# D-3911 | |
| Chemical compound, drug | PP2 | Calbiochem | Cat# 529573 | 1 µM for functional blocking |
| Chemical compound, drug | PP3 | Calbiochem | Cat# 529574 | 1 µM for functional blocking |
| Recombinant DNA reagent | UtrCH-mCherry plasmid | Burkel et al., 2007 | ||
| Recombinant DNA reagent | hVEGFR2-GFP fusion plasmid | |||
| Commercial assay or kit | VEGF ELISA kit | R and D | Cat# MMV00 |