Table 3.
Top 70 CPPs’ secondary structure prediction after conjugation with CPG2.
| Peptide Number | CPPs’ Names | N-Terminal Conjugation with CPG2 | C-Terminal Conjugation with CPG2 | ||
|---|---|---|---|---|---|
| Secondary Structure after Conjugation with CPG2 (C: coil, S: Sheet, H: Helix) |
Percentage of Amino Acids with Helix and Sheet Configuration | Secondary Structure after Conjugation with CPG2 (C: coil, S: sheet, H: helix) |
Percentage of Amino Acids with Helix and Sheet Configuration | ||
| 1 | Transportan 10 (TP10) | CCSSSSSCHHHHCCCCCHHHC | 57.1 | CCCCCCCCCCHHHHHHHHHCC | 42.9 |
| 2 | Ala43 substitution mutant of pAntp (43–58) | CCHHHHHHHCCCCCHH | 56.2 | HHHHHHHHHHHHHHCC | 87.5 |
| 3 | Crot (27–39) derivative 1 | CSSSSSHHHH | 90.0 | CCCSSSSCCC | 40.0 |
| 4 | Crot (27–39) derivative 2 | CSSSSSSHHH | 90.0 | CCCSSSSCCC | 40.0 |
| 5 | Crot (27–39) derivative 3 | CSSSSSSCHH | 80.0 | CCHHHSSCCC | 50.0 |
| 6 | CyLoP-1 | CSSSHHHHHH | 90.0 | CCCCCCCCCC | 0.0 |
| 7 | Crot (27–39) derivative 4 | CCCCCCCCCCH | 9.1 | CCCCCCCCCCC | 0.0 |
| 8 | Crot (27–39) derivative 5 | CCSSSSHHHHH | 81.8 | CHHHHHHCCCC | 54.5 |
| 9 | pAntp (49–58) | CCCCHHHHHH | 60.0 | CCCCCCCCCC | 0.0 |
| 10 | Tat (48–60) | CCCCCCCCCCCCC | 0.0 | CCCCCCCCCCCCC | 0.0 |
| 11 | pAntp (45–58) | CCSSSCCCCCCCHH | 35.7 | CCCCCCCCCCCCCC | 0.0 |
| 12 | Bip15 | CCCCC | 0.0 | CCCCC | 0.0 |
| 13 | pAntp (47–58) | CCCCCCCCCCHH | 16.7 | CCCCCCCCCCCC | 0.0 |
| 14 | II | CHHHHHHHHHHHHHHHHH | 94.4 | HHHHHHHHHHHHHHHHCC | 88.9 |
| 15 | Crot (27–39) derivative 6 | CCCCCCCCCC | 0.0 | CCCCCCCCCC | 0.0 |
| 16 | Crot (27–39) derivative 7 | CCCCCCCCCCCCCH | 7.1 | CCHHHHHHHCCCCC | 50.0 |
| 17 | Crot (27–39) derivative 8 | CCSSSSSCHHH | 27.3 | CCCCCCCCCCC | 0.0 |
| 18 | Crot (27–39) derivative 9 | CCCSSSSHHHHH | 41.7 | CCCCCCCHHCCC | 16.7 |
| 19 | pAntp (46–58) | CCCCCCCCCCCHH | 15.4 | CCCCCHHHHHHCC | 46.2 |
| 20 | 7 | CCCSSSCCCCCCCCH | 26.7 | CCCCCCCCCCCCCCC | 0.0 |
| 21 | No.14–2 | CHHHHHHHHHHHCCC | 73.3 | HHHCCCCHHHHCCCC | 46.7 |
| 22 | No.14 | CCSSSSSCCCCCCHH | 53.3 | CCCCCCCCCCCCCCC | 0.0 |
| 23 | No.14–7 | CCHHHHHCCCCHHHH | 60.0 | CCCCCCCCCCCCCCC | 0.0 |
| 24 | Crot (27–39) | CCCSSSSSHHHHH | 76.9 | CCCCCHHHHHCCC | 38.5 |
| 25 | Crot (27–39) derivative 10 | CCCCSSHHHHHH | 66.7 | CCCCCCCCCCCC | 0.0 |
| 26 | Crot (27–39) derivative 11 | CCCCCCSSCCCHH | 30.8 | CCCCCCCCCCCCC | 0.0 |
| 27 | Crot (27–39) derivative 12 | CCCSSSSHHHHHH | 76.9 | CCCCHHHHHHCCC | 46.2 |
| 28 | pVEC mutant 1 | CHHHHHHHHHCCCCCCCH | 55.5 | CCHHHHHHHHHHHHCCCC | 66.7 |
| 29 | pVEC mutant 2 | CCSSSHHHHHCCCCCCCC | 44.4 | HHHHHHHHHHHCCCCCCC | 61.1 |
| 30 | pVEC mutant 3 | CSSSHHHHHHHCCCCCCH | 61.1 | CCHHHHHHHHHHHCCCCC | 61.1 |
| 31 | Crot (27–39) derivative 13 | CCCSSSSHHHHH | 75.0 | CCCCHHHHHCCC | 41.7 |
| 32 | ARF(1–22) | CCCHHHHHHHHHHHHCCCCCCC | 54.5 | HHHHHHHHHHHCCCCCCCCCCC | 50.0 |
| 33 | M918 | CCSSHHHHHHHHHHCCCCCCCC | 54.5 | CCHHHHHHHHCCCCCCCCCCCC | 36.4 |
| 34 | pAntp (51–58) | CCCCCCHH | 25.0 | CCCCCCCC | 0.0 |
| 35 | pAntp (44–58) | CCCHHHHCCCCCCHH | 40.0 | HHHHHHHHHCCCCCC | 60.0 |
| 36 | pAntp (50–58) | CCCCCCCHH | 22.2 | CCCCCCCCC | 0.0 |
| 37 | Ala44 substitution mutant of pAntp (43–58) | CCCCHHHCCCCCCCHH | 31.2 | HHHHHHHHCCHHHCCC | 68.8 |
| 38 | PDX -1-PTD | CCCHHHHCCCCCCCHH | 37.5 | HHHHHHHHHHHCCCCC | 68.8 |
| 39 | No.14–25 | CSSSSSCCCHHHHHH | 73.3 | CHHCCCCCCCCCCCC | 13.3 |
| 40 | No.14–17 | CSSSSCCCCCCHHHH | 53.3 | HHHHCCCCCCCCCCC | 26.7 |
| 41 | No.14–18 | CCSSSSSCCCCCCCC | 33.3 | HHHHHHHCCCCCCCC | 46.7 |
| 42 | No.14–20 | CCCCCCCCCCCCCCH | 6.7 | CCCCCCCCCCCCCCC | 0.0 |
| 43 | No.14–21 | CCSSSSSSCCCHHHH | 66.7 | HHHHHHHHHHHHHCC | 86.7 |
| 44 | No.14–1135 | CCSSSSSCCCCCCCC | 33.3 | CCCCCCCCCCCCCCC | 0.0 |
| 45 | No.14–1 | CCCSSSCCCCCCCHH | 33.3 | CCCCCCCCCCCCCCC | 0.0 |
| 46 | 30 | CCCSSSCCCHHHHCC | 46.7 | CCCCCCCCCCCCCCC | 0.0 |
| 47 | Ala45 substitution mutant of pAntp (43–58) | CCCSSSSCCCCCCCHH | 37.5 | HHHHHHHHHHHHHCCC | 81.3 |
| 48 | Ala46 substitution mutant of pAntp (43–58) | CCSSSSSCCCCCCCHH | 43.7 | HHHHHHHCCCHHHHCC | 68.8 |
| 49 | Ala47 substitution mutant of pAntp (43–58) | CCHHHCCCCCCCCCHH | 31.2 | HHHHHHHHHHCCCCCC | 62.5 |
| 50 | Ala48 substitution mutant of pAntp (43–58) | CCHHHHHHHCCCCCCC | 43.7 | CCCCHHHCHHHHHCCC | 50.0 |
| 51 | Ala49 substitution mutant of pAntp (43–58) | CCCSSCCCCCCCCCHH | 25 | HHCCHHHHCCCHHCCC | 50.0 |
| 52 | Ala50 substitution mutant of pAntp (43–58) | CCSSSSSCCCCCCCHH | 43.7 | HHHHHHHHHHHHCCCC | 87.5 |
| 53 | pAntpHD (Pro50) | CCCCCCCCCCCCCCHH | 12.5 | HHHHHHCCCCHHHCCC | 68.8 |
| 54 | Ala51 substitution mutant of pAntp (43–58) | CCCSSSSCCCCCCCCH | 31.2 | CCCCCCHHHHHHHHCC | 50.0 |
| 55 | Ala52 substitution mutant of pAntp(43–58) | CCHHHHHHHHHHHCCC | 68.7 | CCCCCHHHCCHHHCCC | 37.5 |
| 56 | Met-Arg | CCHHHHHHHHHHHHHH | 87.5 | HHHHHHHHHHHHHHCC | 87.5 |
| 57 | Ala54 substitution mutant of pAntp(43–58) | CSSSHHHCCCCCCCHH | 50.0 | HHHHHHHHCCHHHHCC | 75.0 |
| 58 | Penetratin | CCSSSSSCCCCCCCCH | 37.5 | HHHHHHHHHHHHHHCC | 87.5 |
| 59 | Retro - Tat (57–49) | CCCCCCCCC | 0.0 | CCCCCCCCC | 0.0 |
| 60 | R6 | CCHHHH | 66.7 | CCCCCC | 0.0 |
| 61 | R9 | CCCCCCCCC | 0.0 | CCCCHHCCC | 22.2 |
| 62 | Crot (27–39) derivative 14 | CSSHHHHHH | 88.9 | CCCSSSCCC | 33.3 |
| 63 | Crot (27–39) derivative 15 | CCSSSHHHHH | 80.0 | CCCCCCCCCC | 0.0 |
| 64 | Rev (34–50) | CCCCCCCCCCCCCCCCC | 0.0 | CCCCCCCCCCCCCCCCC | 0.0 |
| 65 | HIV-1 Rev (34–50) | CCCCCCCCCHHHHHHHCCC | 36.8 | CCCCCCCCCCCCCCCCCCC | 0.0 |
| 66 | Bip6 | CCCCC | 0.0 | CCCCC | 0.0 |
| 67 | Bip1 | CCCCC | 0.0 | CCCCC | 0.0 |
| 68 | Bip2 | CCCCC | 0.0 | CCCCC | 0.0 |
| 69 | Bip16 | CCCCC | 0.0 | CCCCC | 0.0 |
| 70 | pAntp (48–58) | CCCCCCCCCHH | 18.2 | CCCCCCCCCCC | 0.0 |