Table 1.

Experimental design and responses results for nanoemulsion process of hemp seed oil.

Test Run	Nano-Emulsion Process Condition (Factors)			Response Variables
	Pea Protein Conc. (%)	Lecithin Conc. (%)	Ultrasound Process Time (min)	Hydrodynamic Diameter z-ave (nm)	Zeta Potential (mV)	Poly-Dispersity Index (pdi)
1	1.4	3	20	220	−13.9	0.233
2	0.8	0	0	806	−18.2	0.787
3	0.8	6	0	803	−22.5	0.717
4	0.2	0	10	270	−24.3	0.440
5	1.4	6	10	314	−23.4	0.459
6	0.2	6	10	215	−27.3	0.261
7c	0.8	3	10	275	−24.3	0.405
8	1.4	3	0	414	−20.3	0.548
9	0.8	6	20	198	−21.6	0.324
10	1.4	0	10	372	−19.4	0.431
11	0.2	3	20	227	−26.9	0.249
12	0.8	0	20	286	−12.5	0.335
13c	0.8	3	10	332	−22.0	0.443
14	0.2	3	0	292	−24.1	0.475
15c	0.8	3	10	343	−24.4	0.483
EMO 1	0.0	5	18	306	−29.9	0.415
EMOP 2	0.4	5	18	209	−27.3	0.239

c: center point; ¹ Optimized emulsion without protein; ² Optimized emulsion with protein.