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Evaluating specificity and toxicity of the confirmed inhibitors. (A) Immunoblot for IDO1 expression in BT549 and SKOV3 lines treated with the inhibitors at 20 µM for 24 h. Tubulin represents a loading control. (B) The effect of 20 µM concentration of the inhibitors on cell viability measured by WST-1 assay and cell growth measured by SRB assay.
