Skip to main content
PLOS ONE logoLink to PLOS ONE
. 2019 Dec 26;14(12):e0227101. doi: 10.1371/journal.pone.0227101

Impact of the change in the antitubercular regimen from three to four drugs on cure and frequency of adverse reactions in tuberculosis patients from Brazil: A retrospective cohort study

María B Arriaga 1,2,3,4,*, Ninfa M C Torres 1,5, Nelia C N Araujo 1,2, Simone C C Caldas 2, Bruno B Andrade 2,3,4,6,7,8,*, Eduardo M Netto 1,2
Editor: Christophe Sola9
PMCID: PMC6932797  PMID: 31877199

Abstract

Background

The Ministry of Health in Brazil included ethambutol in the intensive phase of sensible tuberculosis (TB) treatment in March 2010, due to the increasing drug resistance, and implemented the fixed dose combination in the TB treatment guidelines.

Methods

A retrospective cohort study was performed to determine the impact of change from three to four drugs schemes on the TB cure and frequency of adverse drug reactions (ADRs) in TB patients. To answer this question, we used data from 730 randomly selected patients who received anti-TB treatment between January 2007 and December 2014 in a reference center from Salvador, Brazil.

Findings

TB patients who received the RHEZ regimen (n = 365) developed ADRs more frequently than those treated with the RHZ (n = 365) (86 [23.6%] vs. 55 [15.1%]; p = 0.01). This difference in ADR incidence was even higher in patients above 30 years-old (64 [74.4%] vs. 36 [65.5%]; p = 0.01). The overall number of ADR episodes was greater in patients from the RHEZ group than in the group that received RHZ (170 [61.4%] vs. 107 [38.6%]; p = 0.03). Multivariable logistic regression analysis adjusted for age, alcohol use and diabetes demonstrated that patients receiving the RHEZ regimen had increased odds of developing ADRs than those undertaking the RHZ scheme (odds ratio [OR]: 1.61, 95% confidence interval [CI]: 1.10–2.35; p = 0.015). The overall cure rate was similar between the distinct treatment groups.

Conclusion

The patients treated with the four-drug regimen exhibited increased risk of ADRs compared to those who received the three-drug regimen, and especially in patients older than 30 years of age.

Introduction

Approximately one quarter of the world's population is estimated to be infected with Mycobacterium tuberculosis, resulting in a major global health problem. In 2016, Brazil reported 2.7/100,000 deaths, and a total incidence of 41/100,000 [1]. Since 1994, the World Health Organization (WHO), together with the International Union Against Tuberculosis and Lung Disease (IUATLD), recommended the use of fixed dose combination (FDC) for TB treatment arguing that this approach simplifies drug dispensing and prevents the development of drug resistance [2]. In 2003, the WHO recommended the addition of ethambutol (E) in basic TB treatment with rifampicin (R), isoniazid (H), and pyrazinamide (Z), due to the lower likelihood of developing drug resistance compared to the other essential drugs [3]. In 2004, a worldwide study on drug resistance performed from 1994–1997 found that, in Brazil, the frequency of a primary isoniazid resistance was 5.9%, whereas of rifampicin was 1.1%, ethambutol 0.1%, streptomycin 6.5% and multidrug resistance was 0.9% [4]. In 2009, the WHO emphasized the previous recommendation, due to even higher rates of drug resistance reported in the late 90’s in countries such as the Dominican Republic (8.6%) and India (13.3%) [5]. At that time, a second national survey on resistance to anti-tuberculosis drugs in Brazil showed an increase in isoniazid resistance alone (from 4.4 to 6.0%) or in its association with rifampicin (1.1 to 1.4%) [6]. For this reason, the Brazilian Ministry of Health, released a technical note changing the treatment regimen from RHZ to RHZE, making ethambutol as the fourth drug in intensive phase treatment, and decreasing the daily doses of isoniazid (from 400mg to 300mg, for an adult weighting more than 50 kg) and pyrazinamide (from 2000mg to 1600mg). Furthermore, the new guidelines changed the four-drugs presentation, combining them into one single tablet called a ‘fixed dose combination’ (RHEZ-FDC) [6,7].

Before the introduction of the new treatment regimen in 2009, a study that included 297 TB patients [8] reported an adverse event rate of 49.1% and 3.7% of patients required a change in therapy due to side effects. A different study in 519 patients from Germany reported an incidence rate of adverse events of 23% [9]. Moreover, the addition of a drug in an already complex therapeutic scheme could potentially increase the frequency of adverse drug reactions (ADRs) requiring interruption of treatment [10], contributing to increases in treatment dropouts and death, particularly in case of drug-induced hepatitis. [11]. Thus, safety of this regimen is a major concern for physicians and patients. After nearly six years after the implementation of the new therapeutic regimen in Brazil, it is necessary to evaluate the such change. The aim of this study was to determine the impact of moving from a three-drug to a four-drug regimen on cure and the frequency of ADR in TB patients from Brazil.

Methods

Ethics statement

All clinical investigations were conducted according to the principles set forth in the Declaration of Helsinki. The Ethics Committee of the Maternidade Climério de Oliveira, Federal University of Bahia (UFBA), approved the study (protocol number: 1.092.460/2015-05). All information given to the research team were de-identified. The Ethics Committee exempted the patient’s informed consent because the data were all from secondary sources.

Design and procedures

The study was conducted with data from patients diagnosed with TB who received anti-TB treatment, between January 2007 and December 2014, at the Brazilian Institute for Tuberculosis Investigation (IBIT). This organization works as a referral center for TB treatment to 13–15% of patients with TB in Salvador/Bahia, city with approximately 3 million inhabitants located in Northeastern Brazil. This center maintains a high-quality standard of treatment and care of TB patients meeting the WHO recommendations on cure and abandonment rates [12]. Doses were administered according to WHO recommendations and Brazilian guidelines based on patient weight [13]

Data on clinical presentation, treatment outcomes, reported ADRs, demographic and laboratory characteristics (biochemical and microbiological) were obtained from medical records (raw data are shown in S1 File). In order to ensure complete data, patient registration and laboratory result logs from IBIT were reviewed. To ascertain accurate information on patients were cross-checked those with the national epidemiological surveillance system in Brazil databases: Notifiable Diseases Information System (Sistema de Informação de Agravos de Notificação–SINAN) and the Mortality Information System (Sistema de Informação de Mortalidade–SIM) [14,15].

Sample size

Sample size was based on the expected 20% of adverse drug reactions rate for RHZ regimen and 30% for those with RHEZ regimen [11,16], assuming 80% power and a 95% confidence level. The estimated number was is 316 participants in each group, with a proportion of 1:1 (before and after drug regimen change) and estimating a safety margin of 15%, the estimated final number was 365 in each group. Randomization was performed using SPSS version 18. The power of the study after collecting data was 83.0%. The calculation of sample size and power were done using open source software, OpenEpi, version 3.01 [17].

Eligibility criteria

The study included patients over 18 years of age, diagnosed with pulmonary or extra pulmonary TB between 2007–2014. The study period was defined based on the following reasons: (i) data from TB patients started being electronically captured at IBIT in 2007; (ii) the new regimen composed by 4-antitubercular drugs was implemented in Brazil by the Ministry of Health on 2014. Patients between January 2007 and February 2010 were included if they started a three-drug regimen [an example is 2R600H400Z20004R600H400/R600H400 for patients over 45 kg (HR in FDC)] and between March 2010 and December 2014 if they started a four-drug regimen (an example is 2E1100Z16004 R600H300/R600H300 for patients over 50 kg (RHEZ in FDC). Patients were ineligible if they were re-treated for TB or had a change in diagnosis during treatment, including a diagnosis of any drug resistance.

Description of adverse drug reactions–ADR

An ADR was defined as "an unintended and harmful reaction to a medication, and which occurs at doses normally used in humans" [18]. An adverse event was defined as "any unfortunate medical event that may occur during treatment with a drug, but does not necessarily have a causal relationship with this treatment" [18]. These two terms were used interchangeably in many cases. However, the final definition of ADR involved a degree of causality with the given treatment. In this paper, the ADRs were described according to the affected organ or system, following the Adverse Reaction Terminology (WHO-ART) [19]. The causality of ADR was assessed using the Naranjo Scale, as improbable = 0, possible = 1–4, probable = 5–8, definitely related ≥ 9 [20]. ADRs were classified as expected if the ADR was listed in the package insert for rifampicin, isoniazid, pyrazinamide and RHEZ-FDC [2123].

Statistical analysis

Demographic and clinical categorical variables were compared using a two-sided Pearson’s chi-square test (Χ2) (with Yates’ correction) or two-tailed Fisher’s exact test among 2X3 or 2x2 tables respectively. The Mantel-Haenszel Test (MH) made for comparison of patients with ADRs between treatment groups. Mean and standard deviation (SD) values were used as measures of central tendency and dispersion. Continuous variables were compared using the student’s t-test, whereas the ANOVA test with Bonferroni post-hoc test was used to compare more than two groups. A multivariable regression model using variables with univariate p-value ≤ 0.2 was performed to assess the odds ratios (OR) and 95% confidence intervals (CIs) of the associations between the occurrence of at least one ADR and the exposure to RHEZ-FDC treatment adjusting for possible confounding factors. The p-values < 0.05 was considered statistically significant. Analyses were all pre-specified and were performed using SPSS version 21.

Results

Between 2007 and 2014, data from patients with diagnosis of pulmonary or extra-pulmonary TB (with microbiological, histopathological or clinical and imaging-based diagnosis) [24,25] were extracted from medical records. After applying the eligibility criteria, there were 769 patients in the RHZ group and 1116 in the RHEZ-FDC group. To avoid sampling bias, we did randomization in order to assign 365 patients to each group (Fig 1).

Fig 1. Study flow diagram.

Fig 1

IBIT: Brazilian Institute for Tuberculosis Investigation (Reference Health Center); RHZ: Rifampicin (600mg), Isoniazid (400mg) and Pyrazinamide (2000mg). Dose to patients >45kg; RHEZ: Rifampicin (150mg), Isoniazid (75mg), Ethambutol (275mg) and Pyrazinamide (400mg) (fixed dose combination-FDC).

Sex, pulmonary TB presentation, diabetes diagnosis, presentation of other comorbidities, concomitant medication use, current or past smoking and alcohol consumption were similar in both groups but mean age for the RHEZ-FDC group was higher than to the RHZ group (40.0 ± 13 years vs. 36.4 ± 13 years; p<0.01). There were no differences in the presentation of patients with MDR-TB between the groups of distinct anti-TB treatment (Table 1).

Table 1. Baseline demographic and clinical presentation of tuberculosis patients.

Characteristics Tuberculosis treatment p-value
RHZ1
(n = 365)
RHEZ-FDC2
(n = 365)
Male sex 208 (57.0) 200 (54.8) 0.68
Age in years 40.0 ± 13 36 ± 13 <0.01
Pulmonary tuberculosis presentation3 336 (92.1) 324 (88.8) 0.24
Diabetes disease at diagnosis 29 (4.9) 33 (9.1) 0.68
Other comorbidities at diagnosis4 28 (7.7) 36 (9.9) 0.30
Concomitant medication5 5 (1.4) 9 (2.5) 0.28
Past or current cigarette smoking6 77 (21.1) 99 (27.1) 0.06
Past or current alcohol consumption7 50 (13.7) 67 (18.4) 0.09
MDR8 16 (4.4) 18 (4.9) 0.86
Patients with ADRs9 55 (15.1) 86 (23.6) 0.01

Data represent no. (%). except age, represented in mean and standard deviation (SD)

1RHZ: Rifampicin (600mg), Isoniazid (400mg) and Pyrazinamide (2000mg). Dose to patients >45kg

2RHEZ: Rifampicin (150mg), Isoniazid (75mg), Ethambutol (275mg) and Pyrazinamide (400mg) (fixed dose combination-FDC). Dose to patients >50kg: 4 FDC.

3Pulmonary plus extra-pulmonary versus extra-pulmonary.

4Other comorbidities at diagnosis: stomach cancer, heart disease, mental disturbance, hypertension, bipolar disorder, psychotic outbreak, schizophrenia, rheumatoid arthritis, lymphedema, asthma, pyogenesis, pleural fibrosis, illicit drug use.

5Concomitant medication: Medicine taken concurrently with treatment anti-tuberculosis.

6Past or current cigarette smoker (daily use) versus no or sporadic use (non-smokers).

7Past or current alcohol consumption: regular (more than 3 days per week) versus no or sporadic–less than 3 days/week (non-drinkers).

8MDR: Multidrug resistance

9ADR: Adverse drug reactions

In this study, we did not classify ADRs by clinical severity, but the interruption of treatment suggested that the ADR was severe. Occurrence of treatment interruption and change in drug regimen schemes caused by ADR were similar between the two groups of patients stratified according to the distinct regimens. Frequency of patients who developed more than 1 episode of ADR was greater in the group of patients treated with RHEZ (53.5%) than in those who were treated with RHZ (32.7%). The causality was reported as possible in 97.1% of ADRs in the RHEZ group vs. 87.9% in the RHZ group (p<0.01) (Table 2).

Table 2. Distribution and characteristics of adverse drug reactions (ADRs) of tuberculosis patients.

ADR distribution and characteristics Tuberculosis treatment p-value
RHZ
n = 55
RHEZ-FDC
n = 86
Patients with > 1 episode–n (%) 18 (32.7) 46 (53.5) <0.01
Treatment intrerruption1 - n (%) 6 (10.9) 8 (9.3) 0.14
Treatment modifications2 - n (%) 2 (3.6) 4 (4.6) 0.79
ADR characteristics3
Expected ADRs—n (%) 99 (92.5) 165 (97.1) 0.08
Causality ADRs–n (%)
Possible 94 (87.9) 165 (97.1) <0.01
Probable 13 (12.1) 4 (2.4) <0.01

1The interruptions were due to toxicity, being two to four days, the minimum and maximum interruption time respectively.

2In the RHZ group, two modifications were for decreased dose isoniazid: 75mg/kg/day. In the group RHEZ, two modifications were for dose of isoniazid: 75mg/kg /day and two decreases dose isoniazid in 75 mg/kg/day and rifampicin at 150 mg/kg/day. All modifications were made after the reintroduction of the scheme with drugs as follows: rifampicin + ethambutol, followed by isoniazid and finally pyrazinamide.

3ADRs total in each group: n = 107 in RHZ group and n = 170 in RHEZ group

ADR was more frequent in patients older than 30 years, especially in those using RHEZ-FDC (64 [74.4%] vs. 36 [65.5%]; p = 0.01) (Table 3). In the RHZ group, we found that 4 (1.1%) patients were simultaneously failure and MDR cases whereas in the RHEZ group, two patients (0.6%) had the co-condition, with no statistical difference between the groups (p = 0.68). Moreover, patients under RHEZ-FDC regimen developed more frequently ADRs than those using RHZ regimen (86 [23.6%] vs. 55 [15.1%]; p = 0.01) (Fig 2A). Cure, transfers, withdrawals and deaths were also similar between the groups (Fig 2B). There was one death in the RHEZ-FDC group related the regimen due to liver failure; this patient had also diabetes, but no history of alcohol or tobacco consumption and the death occurred after 18 doses TB treatment. Another patient was hospitalized within 3 days due to the presentation of pruritus, vomiting diarrhea and melena; his clinical presentation was controlled during hospitalization and TB treatment resulted in microbiological and clinical cure.

Table 3. Treatment outcome and sputum smear result of tuberculosis patients.

Characteristics Tuberculosis treatment  
RHZ RHEZ-FDC
ADR
n = 55
No ADR
n = 310
p-value ADR
n = 86
No ADR
n = 279
p-value p-value*
Age in years** 35 ± 11 37 ± 13 0.32 41 ± 12 39 ± 13 0.02 0.08
Range age ≥30 years 36 (65.5) 191 (61.6) 0.65 64 (74.4) 197 (70.6) 0.005 0.01
Female 32 (58.2) 125 (40.3) 47 (54.7) 118 (42.3)
Pulmonary TB presentation 50 (90.9) 286 (92.3) 0.02 76 (88.4) 248 (88.9) 0.048 0.002
Treatment outcome 0.42 0.55 0.41
Cure1 47 (85.5) 266 (85.8) 75 (87.2) 251 (90)
Failure2 0 (0) 3 (1) 0 (0) 0 (0)
Not evaluated3 1 (1.7) 0 (0) 3(3.5) 0 (0)
Death4 3 (5.5) 6 (1.9) 2 (2.3) 5 (1.8)
Lost to follow-up5 4 (7.3) 20 (6.5) 6 (7) 10 (3.6)
MDR6 1 (1.8) 15 (4.8) 0.48 3 (3.5) 13 (4.7) 0.78 0.49
Diabetes 4 (7.3) 25 (8.1) 1.00 14 (16.3) 19 (6.8) 0.16 0.06
Hypertension 1 (1.8) 1 (0.3) 0.28 2 (2.3) 3 (1.1) 0.34 0.14
Past or current alcohol consumption 18 (33.8) 32 (2.3) <0.01 15 (17.4) 52 (18.6) 0.78 0.08
Past or current cigarette smoking 19 (34.5) 58 (18.7) 0.02 25 (29.1) 74 (26.5) 0.78 0.09
Illicit drug use 1 (1.8) 5 (1.6) 1.00 3 (3.5) 4 (1.4) 0.38 0.29
Concomitant medication 3 (5.5) 2 (0.6) 0.03 3 (3.5) 6 (2.2) 0.46 0.04

Data represent no. (%), except age, represented in mean and standard deviation (SD)

* Mantel-Haenszel Test (MH) for comparison of patients with ADRs between treatment groups

** ANOVA for com for comparison of patients with ADRs between treatment groups

1Cured: A pulmonary TB patient with bacteriologically confirmed TB at the beginning of treatment who was smear- or culture-negative in the last month of treatment and on at least one previous occasion.

2Failure: Persistence of positive sputum at the end of treatment also classified as failure cases that at the beginning of treatment, are strongly positive (++ or +++) and maintain this situation until the fourth month, or those with initial positivity followed by negativity, and new positivity for two consecutive months, from the fourth month treatment.

3Not evaluated: A TB patient for whom no treatment outcome is assigned. This includes cases “transferred out” to another treatment unit.

4Death: A TB patient who dies for any reason before starting or during the course of treatment

5Lost to follow-up: A TB patient who did not start treatment or whose treatment was interrupted for two consecutive months or more

6MDR: Multidrug-resistant tuberculosis

Fig 2. Distribution of ADRs in TB patients.

Fig 2

(A) Frequency of ADRs in patients with RHZ and RHEZ anti- TB regime. (B) Outcomes treatment were compared between patients with RHZ and RHEZ anti-TB treatment. The significant p values are shown. (C) ADRs frequencies of anti-TB treatment groups with RHZ and RHEZ are shown. (D) ADRs distribution for systems were compared between patients with RHZ and RHEZ anti-TB treatment. The significant p-values are shown. Others: Hepatic, ocular, circulatory and respiratory system.

The number of ADR episodes was greater in the four-drug regimen compared to three-drug group (170 [43.3%] vs. 107 [25.8%], respectively; Fig 2C). The most affected organ system in both groups was gastrointestinal (RHEZ-FDC [27.6%] vs. RHZ [35.5%]; p = 0.17), followed by skin and appendages (RHEZ-FDC [24.1%] vs. RHZ [15.0%]; p = 0.07) (Fig 2D).

The total number of cases MDR per year in Brazil increased between 2007 to 2014. In Salvador, a similar trend happened until 2014, where there was a decrease. At IBIT, the year 2014 presented the highest number of MDR cases (8 cases) between 2007 and 2014 (Fig 3A). Multivariable regression analysis revealed that TB patients with RHEZ-FDC treatment had 1.61 (95%CI: 1.10–2.35) times greater odds to have at least one adverse event (Fig 3A), after adjustment for age (≥ 30 years), alcohol consumption and diabetes (Fig 3B).

Fig 3. MDR-TB cases and crude and odds ratio for adverse drug reaction in tuberculosis patients.

Fig 3

(A) Total number of MDR-TB cases shown per year in Brazil, Salvador and IBIT (B) A multivariable regression model adjusted for RHEZ-FDC treatment, age ≥ 30 years, alcohol consumption and diabetes. The odds associated with the covariates used in the model adjustment are displayed in Table 3. OR = Odds ratio; CI = confidence intervals.

Discussion

Although it is critical to assess the pros and cons of the implementation of the antitubercular treatment with RHEZ-FDC, there has no previous study directly comparing the current RHEZ-FDC therapy regimen with the previously used RHZ. The present study adds to the current knowledge in the field as it shows that the ADRs incidence increased after introduction of the fourth drug regimen in patients being treated for TB and this increase was more commonly observed after the third decade of age.

The proportion of participants presenting with at least one ADR event in this study was lower (24.2%) than what has been published by other studies performed in India [26], that reported an incidence of 74.1% and in Pakistan [27], in which the incidence reached up to 40.0%. In Brazil, a global incidence of patients with ADR is thought to be around 47% [28]. In our study, the frequency of ADRs within those under 30 years of age was similar in both groups, but the frequency increased significantly in the RHEZ-FDC group among those over 30 years of age, compared to that in those who received RHZ-based therapy. While there is evidence to support the idea that ADR events may be more frequent in TB patients proportional to age [29], other studies found no association between increasing age and risk of adverse reactions [30]. The latter study justified its results speculating that physicians prescribed more often hepatoprotective drugs, such as N-acetyl-cysteine, during the treatment of elderly patients. Patients included in the group from 2010 to 2014 and who were treated with the four-drug regimen were significantly older compared with those from the previous years and who received three drugs. This difference could be attributed to the accelerated aging of the Brazilian population, also described in people with TB in those years [31]. Furthermore, epidemiological factors such as TB and HIV control programs could have impacted the TB prevalence in age groups below 30 years old [32].

Our findings indicate that the most common body systems affected by the four-drug regimen were the gastrointestinal tract and neurological. Hence, the most frequent ADR was vomiting, which can be considered from mild to moderate, and managed symptomatically without interruption of anti TB treatment [33]. In the RHZ group, the most affected system were gastrointestinal and neurological and for RHEZ -FDC group was the skin and appendages, which is expected due to the use of pyrazinamide and ethambutol, that are responsible for skin reactions [34]. Our results differ from those reported in a study from China [30], which found that the most frequent ADRs occurred in the liver, representing 9.3% of all the ADRs, while, in our study, the incidence of liver-related ADR was less than 1%. This result could be attributed to underreporting of ADRs in our study, and that not all patients had laboratory results of the liver function tests during treatment.

Previous studies have shown that alcohol and tobacco consumption may predispose and accelerate hepatotoxic effects, caused especially by isoniazid [29,30]. In the present study, we found that the regimen with RHEZ was strongly associated with occurrence of at least one ADR event independent of other confounding factors. Nevertheless, risk variables such as malnutrition or history of liver problems could not be obtained since the data were not recorded in all cases.

Of note, herein no ADR event was classified as definitive. Most of the ADRs have been previously described in the medication’s package insert and only 1.9% required the discontinuation of treatment as a result of the ADR, which was lower than what it has been reported by a similar study [35]. In this context, the presentation of ADR becomes a challenge for TB control that has not yet been completely deluded. It is currently known that there are crucial factors associated with ADRs, such as genetic variants, which influence the effectiveness of treatment and the presentation of ADR [36,37]. Enzymes that metabolize anti-TB drugs can undergo genetic or epigenetic alterations, which may result in reduced or complete loss of activity, thus causing toxic damage to cells [36,37].

RHEZ-FDC have been implemented for those under basic drug-sensitive anti-tuberculosis treatment regimen, to both reduce the number of MDR-TB cases and decrease poor outcomes of treatment [38]. However, there was no major change in the number cases MDR-TB in Brazil at the time of implementation of the new four-drug regimen. Although FDC was recommended by the WHO and IUTLD to reduce emergence of drug resistance, other studies have suggested that the use of FDC is related to the risk of treatment failure or TB recurrence, depending on the bioavailability of drugs [2]. Thus, the fact that FDC implementation had no effect on the change in MDR-TB cases in Brazil could have been due to the implementation of fixed-dose use in anti-TB treatment without the evaluation of the bioavailability of drugs, especially rifampicin, as emphasized when the recommendation was published [2]. Furthermore, the addition of ethambutol to the RHZ-FDC scheme could have reduced the risk of additional resistance to isoniazid monoresistance, rifampin or simultaneous resistance to isoniazid and pyrazinamide, but not in cases of simultaneous resistance to isoniazid and rifampicin, which defines MDR cases [39]. Our results together with those of other studies [11,39] suggest the great need to perform the bioavailability assessment and also the sensitivity tests during implementation of FDC.

Our results show differences in the trend of reported cases such as MDR-TB between the Salvador city and IBIT through years 2007–2014, this could be explained by the holistic management, performed at IBIT with services offered in conjunction with specialized mental health centers and psychosocial care centers as well as continuous patient support [12].

This study shows that failure outcomes in both treatments were similar, but this study was not designed to assess outcomes of anti-TB treatment. It is most probably too early to observe the effect on efficacy however, the efficacy of RHEZ-FDC has not yet been fully established as observed in a recent systematic review [40].

There are several limitations in our study. When using secondary data, these may be inaccurate. As indicated earlier, the patients in this study were treated with several medications, therefore, it is difficult to analyze the causality between the presentation of ADR and the specific medications separately. Another important point is that not all participants attended monthly clinical evaluations or were not tested in the laboratory during treatment, which could carry out a sub-notification of ADR or not obtain more information to establish the intensity/severity of ADR.

In conclusion, our findings indicated that the risk of ADR is likely more frequent in TB patients receiving RHEZ-FDC compared to that in those undertaking RHZ. This difference was more striking among those over 30 years of age. Such high-risk patient subpopulation should be followed up more carefully when the 4-drug regimen is being used [41].

Supporting information

S1 File. De-identified raw data extracted from the medical records.

(XLSX)

Acknowledgments

We thank Fundação José Silveira for the support.

Data Availability

All relevant data are within the paper and its Supporting Information files.

Funding Statement

This study was supported by the Intramural Research Program from Fundação José Silveira, Universidade Federal da Bahia and Program of Fundação Oswaldo Cruz (FIOCRUZ). BBA is a senior researcher from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). MBA received a fellowship from the Fundação de Amparo à Pesquisa da Bahia (FAPESB). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

  • 1.World Health Organization Global Tuberculosis Programme G. Global Tuberculosis Report 2016. Geneva; 2016.
  • 2.International Union Against Tuberculosis and Lung Diseases (IUATLD), WHO Tuberculosis Programme. The promise and reality of fixed-dose combinations with rifampicin. Int Union Against Tuberc Lung Dis. 1994;69: 219–220. [DOI] [PubMed] [Google Scholar]
  • 3.World Health Organization Global Tuberculosis Programme G. Treatment of Tuberculosis Guidelines for National Programmes [Internet]. 2003 [cited 19 Sep 2016]. Available: http://apps.who.int/iris/bitstream/10665/67890/1/WHO_CDS_TB_2003.313_eng.pdf
  • 4.World Health Organization / International Union Against Tuberculosis and Lung Disease Global Project on Anti-tuberculosis Drug Resistance Surveillence 1994–1997. Anti-Tuberculosis Drug Resistance in the World Report No. 1. Geneva; 1997.
  • 5.World Health Organization Global Tuberculosis Programme G. Global Tuberculosis Report 2009. Geneva; 2009.
  • 6.Nacional P, Inqu II. Nota técnica sobre as mudanças no tratamento da tuberculose no Brasil para adultos e adolescentes. 2009.
  • 7.BRASIL M da S do. Nota técnica sobre as mudanças no tratamento da tuberculose no Brasil para adultos e adolescentes [Internet]. 2009 p. 5.
  • 8.Vieira DEO, Gomes M. Efeitos adversos no tratamento da tuberculose: experiência em serviço ambulatorial de um hospital-escola na cidade de São Paulo. J Bras Pneumol. 2008;34: 1049–1055. 10.1590/s1806-37132008001200010 [DOI] [PubMed] [Google Scholar]
  • 9.Schaberg T, Rebhan K, Lode H. Risk factors for side-effects of isoniazid, rifampin and pyrazinamide in patients hospitalized for pulmonary tuberculosis. Eur Respir J. 1996;9: 2026–2030. 10.1183/09031936.96.09102026 . [DOI] [PubMed] [Google Scholar]
  • 10.Forget EJ, Menzies D. Adverse reactions to first-line antituberculosis drugs. Expert Opin Drug Saf. 2006;5: 231–249. [PubMed: 10.1517/14740338.5.2.231 ]. [DOI] [PubMed] [Google Scholar]
  • 11.Marra F, Marra C a, Bruchet N, Richardson K, Moadebi S, Elwood RK, et al. Adverse drug reactions associated with first-line anti-tuberculosis drug regimens. Int J Tuberc Lung Dis. 2007;11: 868–875. [PubMed: ]. [PubMed] [Google Scholar]
  • 12.Slatery E. Neglected or Non-compliant? Assessing the difficulties of tuberculosis patients in Salvador-BA, Brazil. Indep Study Proj Collect. 2014; 1–45. Available: http://digitalcollections.sit.edu/isp_collection/1944 [Google Scholar]
  • 13.Nacional P, Devep T. Mudanças no tratamento da tuberculose no Brasil [Internet]. [cited 27 Jul 2016]. Available: http://www.saude.rs.gov.br/upload/1339785741_MudançasnoTratamentodaTuberculose—apresentaçãoparaareuniãodemultiplicadores.pdf
  • 14.DATASUS—SIM—Sistema de Informação sobre Mortalidade [Internet]. [cited 28 Nov 2016]. Available: http://sim.saude.gov.br/default.asp
  • 15.SINAN—Sistema de Informação de Agravos de Notificação [Internet]. [cited 28 Nov 2016]. Available: http://sinan.saude.gov.br/sinan/login/login.jsf
  • 16.Diretoria de Vigilância Epidemiológica. Boletim Epidemiológico Avanços no Diagnóstico da Tuberculose [Internet]. Brasília; 2015. pp. 1–4. Available: http://portalsaude.saude.gov.br/index.php/o-ministerio/principal/leia-mais-o-ministerio/197-secretaria-svs/11955-boletins-epidemiologicos-arquivos
  • 17.Sullivan KM, Dean A. OPENEPI: A Web-based epidemiologic and statistical calculator for Public Health. 2009;124: 471–474. 10.1177/003335490912400320 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.World Health Organization—Geneva. Safety Monitoring of Medicinal Products [Internet]. 2012 [cited 25 Sep 2016]. Available: http://apps.who.int/medicinedocs/documents/s19132en/s19132en.pdf
  • 19.World Health Organization—Geneva. The WHO Adverse Reaction Terminology–WHO-ART [Internet]. 2015 [cited 18 Sep 2016]. Available: http://www.who.int/hiv/topics/pharmacovigilance/2_who_art.pdf
  • 20.Naranjo et al. A method for estimating the probability of adverse drug reactions. Clin Pharmacol Ther. 1981; 239–245. 10.1038/clpt.1981.154 [DOI] [PubMed] [Google Scholar]
  • 21.World Health Organization. WHOPAR TB158 [Internet]. [cited 26 Nov 2016]. Available: http://apps.who.int/prequal/WHOPAR/WHOPARPRODUCTS/WHOPAR_TB158.htm
  • 22.World Health Organization. WHOPAR TB168 [Internet]. [cited 26 Nov 2016]. Available: http://apps.who.int/prequal/WHOPAR/WHOPARPRODUCTS/WHOPAR_TB168.htm
  • 23.World Health Organization. WHO List of Prequalified Medicinal Products [Internet]. [cited 11 Nov 2016]. Available: http://apps.who.int/prequal/query/ProductRegistry.aspx
  • 24.National Institute for Health and Care Excellence. Tuberculosis [Internet]. United Kingdom; 2016 [cited 25 Sep 2016] pp. 1–178. Available: https://www.nice.org.uk/guidance/ng33/resources/tuberculosis-1837390683589
  • 25.Nahid P, Dorman SE, Alipanah N, Barry PM, Brozek JL, Cattamanchi A, et al. Executive Summary: Official American Thoracic Society/Centers for Disease Control and Prevention/Infectious Diseases Society of America Clinical Practice Guidelines: Treatment of Drug-Susceptible Tuberculosis. Clin Infect Dis. 2016;63: 853–867. 10.1093/cid/ciw566 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Khade Amitkumar Anandrao and Vishal Janu Gothal K. Incidence and comparative analysis of adverse drug reactions in DOTS. Int J Pharm Sci Res. 2013;4: 3641–3644. 10.13040/IJPSR.0975-8232.4(9).3641-44 [DOI] [Google Scholar]
  • 27.Nazir A, Imam SF, Shabbir I, Saleem S. Adverse Drug Reactions of First Line Anti-tuberculosis Drugs used in DOTS Implemented Setting in Lahore. Pak J Med Res. 2015;54: 101–105. Available: http://applications.emro.who.int/imemrf/Pak_J_Med_Res/Pak_J_Med_Res_2015_54_4_101_104.pdf [Google Scholar]
  • 28.Maciel ELN, Guidoni LM, Favero JL, Hadad DJ, Molino LP, Jonhson JL, et al. Adverse effects of the new tuberculosis treatment regimen recommended by the Brazilian Ministry of Health. J Bras Pneumol. 36: 232–238. [PubMed: 10.1590/s1806-37132010000200012 ]. [DOI] [PubMed] [Google Scholar]
  • 29.Chung-Delgado K, Revilla-Montag A, Guillen-Bravo S, Velez-Segovia E, Soria-Montoya A, Nuñez-Garbin A, et al. Factors associated with anti-tuberculosis medication adverse effects: A case-control study in Lima, Peru. PLoS One. 2011;6: 1–6. 10.1371/journal.pone.0027610 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Zhang T, Du J, Yin X, Xue F, Liu Y, Li R, et al. Adverse Events in Treating Smear-Positive Tuberculosis Patients in China. Int J Environ Res Public Health. 2015;13: 1–11. [PubMed: 10.3390/ijerph13010086 ]. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.IBGE:: Instituto Brasileiro de Geografia e Estatística. Projeção da populacão do Brasil pelo sexo e idade: 2000–2060 [Internet]. [cited 25 Sep 2016]. Available: http://www.ibge.gov.br/espanhol/estatistica/populacao/projecao_da_populacao/2013/default.shtm
  • 32.Gaspar RS, Nunes N, Nunes M, Rodrigues VP. Temporal analysis of reported cases of tuberculosis and of tuberculosis-HIV co-infection in Brazil between 2002 and 2012. J Bras Pneumol. 2016;42: 416–422. 10.1590/S1806-37562016000000054 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Shin HJ, Kwon YS. Treatment of drug susceptible pulmonary tuberculosis. Tuberc Respir Dis (Seoul). 2015;78: 161–167. 10.4046/trd.2015.78.3.161 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Sadiq S, Khajuria V, Tandon VR, Mahajan A, Singh JB. Adverse Drug Reaction Profile in Patients on Anti-tubercular Treatment Alone and in Combination with Highly Active Antiretroviral Therapy. J Clin diagnostic Res. 2015;9: FC01–FC04. [PubMed: 4625257]. 10.7860/JCDR/2015/13452.6652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 35.Resende LSO, Dos Santos-Neto ET. Risk factors associated with adverse reactions to antituberculosis drugs. J Bras Pneumol. 2015;41: 77–89. 10.1590/S1806-37132015000100010 . [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Sahu R, Singh K, Subodh S. Adverse Drug Reactions to Anti-TB Drugs: Pharmacogenomics Perspective for Identification of Host Genetic Markers. Curr Drug Metab. 2015;16: 538–552. 10.2174/1389200216666150812123725 [DOI] [PubMed] [Google Scholar]
  • 37.Zeng J, Xie L, Luo H, Xie J. The epigenetic modifications of genes associated with tuberculosis susceptibility and implications for Epi-drugs. Crit Rev Eukaryot Gene Expr. 2015;25: 349–362. 10.1615/critreveukaryotgeneexpr.2015014334 [DOI] [PubMed] [Google Scholar]
  • 38.Blomberg B, Spinaci S, Fourie B, Laing R. The rationale for recommending fixed-dose combination tablets for treatment of tuberculosis. Bull World Health Organ. 2001;79: 61–68. [PubMed: ]. S0042-96862001000100012 [pii] [PMC free article] [PubMed] [Google Scholar]
  • 39.Mitchison DA. Infectivity of patients with pulmonary tuberculosis during chemotherapy. Eur Respir J. 1990; 385–386. [PubMed] [Google Scholar]
  • 40.Gallardo Carmen R, Rigau Comas D, Valderrama Rodríguez A, Roqué i Figuls M, Parker Lucy A, Caylà J, et al. Fixed-dose combinations of drugs versus single drug formulations for treating pulmonary tuberculosis. Cochrane Database Syst Rev. 2016; 1–147 [PubMed: 27186634] 10.1002/14651858.CD012199 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 41.Albanna AS, Smith BM, Cowan D, Menzies D. Fixed-dose combination antituberculosis therapy: a systematic review and meta-analysis. Eur Respir J. 2013;42: 721–732. [PubMed: 10.1183/09031936.00180612 ]. [DOI] [PubMed] [Google Scholar]

Decision Letter 0

Christophe Sola

6 Nov 2019

PONE-D-19-25724

Impact of the change in the antitubercular regimen from three to four drugs on cure and frequency of adverse reactions in tuberculosis patients from Brazil: a retrospective cohort study

PLOS ONE

Dear Dr. Andrade,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

==============================

Please see list of suggestions below, please provide a revised paper with a point by point rebuttal letter

==============================

We would appreciate receiving your revised manuscript by Dec 21 2019 11:59PM. When you are ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter.

To enhance the reproducibility of your results, we recommend that if applicable you deposit your laboratory protocols in protocols.io, where a protocol can be assigned its own identifier (DOI) such that it can be cited independently in the future. For instructions see: http://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). This letter should be uploaded as separate file and labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. This file should be uploaded as separate file and labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. This file should be uploaded as separate file and labeled 'Manuscript'.

Please note while forming your response, if your article is accepted, you may have the opportunity to make the peer review history publicly available. The record will include editor decision letters (with reviews) and your responses to reviewer comments. If eligible, we will contact you to opt in or out.

We look forward to receiving your revised manuscript.

Kind regards,

Christophe Sola, Pharm.D., Ph.D.

Academic Editor

PLOS ONE

Journal Requirements:

When submitting your revision, we need you to address these additional requirements.

Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at

http://www.journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and http://www.journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

1. Please refer to any post-hoc corrections made during your statistical analysis. Please justify the reasons if these were not performed. Additionally, please thoroughly discuss any limitations of your study within the Discussion section.

2. Please include a copy of Table 4 which you refer to in your text on page 10

Additional Editor Comments (if provided):

PONE-D-19-25724 Review reviewer 1

In this work Arriaga et al report changes occurring in the overall outcome of patients and in the frequency of adverse reactions subsequent to the modification of the antitubercular regimen from three to four drugs in Brazil. This a retrospective cohort study which enrolled 365 patients treated with three anti-TB drug regimen (RHZ) from the Jan 2007 to Feb 2010 and 365 patients treated with four anti-TB drug regimen (RHEZ) from Mar 2010 to Dec 2014.

The main results are an increase in the adverse effects which were higher in the RHEZ (23.6%) vs RHZ (15.1%), especially in individuals > 30 years. There were no significant differences between the two groups regarding the outcome (cure or failure), though the authors stress the fact that the study was not designed to point to such differences.

Altogether this is an interesting report on the consequences of the adjustment of the standard recommended anti-TB regimen, capturing an extended period of time and a large retrospective cohort. However, several aspects need clarification prior to publication.

Comments:

1. Page 3 line 46: isoniazid instead of rifampicin

2. Adverse effects (ADR) were higher in the RHEZ (23.6%) vs RHZ (15.1%), especially in individuals > 30 years. However, it should be stressed that severe ADR leading to either interruption or treatment modification was similar between the two regimens.

3. Though authors indicate that failure rates were unchanged, failure seems to have occurred only in the RHZ group. Could the authors clarify this issue? Were the failures related to resistance emergence?

4. Are data regarding the evolution of isoniazid mono-resistance concomitant with the regimen change available?

5. Which were the neurological side effects observed? Was-it ethambutol-induced optic neuropathy?(less...)

PONE-D-19-25724 Review reviewer 2

General comments

This retrospective study assess the ADR of two drug regimens used for tuberculosis treatment. It is a topic of high societal interest, done on a significant number of patients, however with some points that could be improved. The English language is not optimal and the paper should be reviewed by a native English speaker. Moreover the discussion could be substantially improved.

Specific comments

“Since 1994, the WHO, together with the International Union Against Tuberculosis and Lung Disease (IUATLD) recommended the use of fixed dose combination (FDC) in treatment of TB because it simplifies drug dispensing and prevents the development of drug resistance [2,3].”

According to the reviewer, this might be an historical mistake: FDC started to be recomemmended by WHO only in 2001 and not in 1994 when TB was declared as World-wide emergency (1993). Please check and provide reference.

Bull World Health Organ. 2001;79(1):61-8. Epub 2003 Nov 5. The rationale for recommending fixed-dose combination tablets for treatment of tuberculosis. Blomberg B1, Spinaci S, Fourie B, Laing R

Lane 55 : please already mention here the most important ADR observed, related to treatment changes.

Lane 111 : please explain if there was a stratification between Adverse events non requiring hospitalization (minor, mild or moderate) from Adverse events requiring hospitalization (major, severe). Comment if not and in particular try to evaluate if some ADR required hospitalization or not, if not mention it.

Lane 145: mean age for the RHEZ-FDC group was higher than to the RHZ group (40.0 ± 13 years vs. 36.4 ± 13 years; p<0.01).

Please explain why, if randomly sampled, there is a significant difference of age between the two groups, in particular comment if this difference could be related to and decrease TB recent transmission rate relative to reactivation in group RHEZ versus to group RHZ.

Lane 196-198 : please comment on the reasons of the observed discrepancy between IBIT figures and Salvador figures

Lane 239. :”field” should replace “filed”

Lane 292 : “though there was no major change in the number cases MDR-TB in Brazil at the time of implementation of the new four-drug regimen”.

I think that this point deserves more discussion, indeed, if the cure rate is not significantly different in the new regimen (point not so well discussed and difficult to estimate on Fig 2B) , if there are more ADR and if MDR-TB rate does not descrease, then what is the true impact of this new regimen as mentioned two lines after ?

The paper also mentions a Table 4 that was not found and the reference to this Table should be suppressed

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

**********

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

**********

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: In this work Arriaga et al report changes occurring in the overall outcome of patients and in the frequency of adverse reactions subsequent to the modification of the antitubercular regimen from three to four drugs in Brazil. This a retrospective cohort study which enrolled 365 patients treated with three anti-TB drug regimen (RHZ) from the Jan 2007 to Feb 2010 and 365 patients treated with four anti-TB drug regimen (RHEZ) from Mar 2010 to Dec 2014.

The main results are an increase in the adverse effects which were higher in the RHEZ (23.6%) vs RHZ (15.1%), especially in individuals > 30 years. There were no significant differences between the two groups regarding the outcome (cure or failure), though the authors stress the fact that the study was not designed to point to such differences.

Altogether this is an interesting report on the consequences of the adjustment of the standard recommended anti-TB regimen, capturing an extended period of time and a large retrospective cohort. However, several aspects need clarification prior to publication.

Comments:

1. Page 3 line 46: isoniazid instead of rifampicin

2. Adverse effects (ADR) were higher in the RHEZ (23.6%) vs RHZ (15.1%), especially in individuals > 30 years. However, it should be stressed that severe ADR leading to either interruption or treatment modification was similar between the two regimens.

3. Though authors indicate that failure rates were unchanged, failure seems to have occurred only in the RHZ group. Could the authors clarify this issue? Were the failures related to resistance emergence?

4. Are data regarding the evolution of isoniazid mono-resistance concomitant with the regimen change available?

5. Which were the neurological side effects observed? Was-it ethambutol-induced optic neuropathy?

**********

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files to be viewed.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email us at figures@plos.org. Please note that Supporting Information files do not need this step.

PLoS One. 2019 Dec 26;14(12):e0227101. doi: 10.1371/journal.pone.0227101.r002

Author response to Decision Letter 0


20 Nov 2019

PONE-D-19-25724 Review reviewer 1

Reviewer #1: In this work Arriaga et al report changes occurring in the overall outcome of patients and in the frequency of adverse reactions subsequent to the modification of the antitubercular regimen from three to four drugs in Brazil. This a retrospective cohort study which enrolled 365 patients treated with three anti-TB drug regimen (RHZ) from the Jan 2007 to Feb 2010 and 365 patients treated with four anti-TB drug regimen (RHEZ) from Mar 2010 to Dec 2014.

The main results are an increase in the adverse effects which were higher in the RHEZ (23.6%) vs RHZ (15.1%), especially in individuals > 30 years. There were no significant differences between the two groups regarding the outcome (cure or failure), though the authors stress the fact that the study was not designed to point to such differences.

Altogether this is an interesting report on the consequences of the adjustment of the standard recommended anti-TB regimen, capturing an extended period of time and a large retrospective cohort. However, several aspects need clarification prior to publication.

Reply: We thank the reviewer for his constructive criticism, which has led to significant improvement of the manuscript.

Comments:

General comments

1. Please refer to any post-hoc corrections made during your statistical analysis. Please justify the reasons if these were not performed.

Reply: Thanks for your observation. The following tests were performed: One-Way ANOVA with Bonferroni post-hoc test was used to compare continuous variables between more than two groups. In addition, Pearson’s chi-square test (with Yates’ correction) or two-tailed Fisher’s exact test was used to compare categorical variables among 2X3 or 2x2 tables respectively. We have now updated the statistical analysis section in the methodology and in the table 3.

2. Additionally, please thoroughly discuss any limitations of your study within the Discussion section.

Reply: The text of the limitations was added to the discussion section.

3. Please include a copy of Table 4 which you refer to in your text on page 10

Reply: The text was supposed to refer to Figure 3B instead of Table 4; it was a typo caused by reformulation of a previous version of the manuscript. We apologize for the typo which has now been corrected.

Additional Editor Comments (if provided):

PONE-D-19-25724 Review reviewer 1

In this work Arriaga et al report changes occurring in the overall outcome of patients and in the frequency of adverse reactions subsequent to the modification of the antitubercular regimen from three to four drugs in Brazil. This a retrospective cohort study which enrolled 365 patients treated with three anti-TB drug regimen (RHZ) from the Jan 2007 to Feb 2010 and 365 patients treated with four anti-TB drug regimen (RHEZ) from Mar 2010 to Dec 2014.

The main results are an increase in the adverse effects which were higher in the RHEZ (23.6%) vs RHZ (15.1%), especially in individuals > 30 years. There were no significant differences between the two groups regarding the outcome (cure or failure), though the authors stress the fact that the study was not designed to point to such differences.

Altogether this is an interesting report on the consequences of the adjustment of the standard recommended anti-TB regimen, capturing an extended period of time and a large retrospective cohort. However, several aspects need clarification prior to publication.

Comments:

1. Page 3 line 46: isoniazid instead of rifampicin

Reply: We have made the indicated change accordingly.

2. Adverse effects (ADR) were higher in the RHEZ (23.6%) vs RHZ (15.1%), especially in individuals > 30 years. However, it should be stressed that severe ADR leading to either interruption or treatment modification was similar between the two regimens.

Reply: The text was updated in results section accordingly.

3. Though authors indicate that failure rates were unchanged, failure seems to have occurred only in the RHZ group. Could the authors clarify this issue? Were the failures related to resistance emergence?

Reply: Yes. A hypothesis is that there is a relationship between occurrence of treatment failure and the emerging number of drug resistant cases. In fact, in the RHZ group, we found that 4 patients were simultaneously failure and MDR cases whereas in the RHEZ two patients had the co-condition. We have added this information to the Results section (xxx).

Are data regarding the evolution of isoniazid mono-resistance concomitant with the regimen change available?

Reply: No was concomitant, since the change was made since March 2010 and the primary resistance to H increased from 4.4% (period 1995-1997) to 6.0% (2007-2008), in Brazil

4. Which were the neurological side effects observed? Was-it ethambutol-induced optic neuropathy?(less...)

Reply: In both groups they presented: migraine, headache and stinging. Only one case and ocular neuropathy occurred in the RHEZ-FD group, but no causality could be established because the patient did not have other clinical evaluations in the center and was transferred to a referral hospital.

We have added the requested information following the reviewer’s recommendation

PONE-D-19-25724 Review reviewer 2

General comments

This retrospective study assess the ADR of two drug regimens used for tuberculosis treatment. It is a topic of high societal interest, done on a significant number of patients, however with some points that could be improved. The English language is not optimal and the paper should be reviewed by a native English speaker. Moreover the discussion could be substantially improved.

Reply: The entire text was reviewed by a native English speaker and the discussion was updated.

Specific comments

1. “Since 1994, the WHO, together with the International Union Against Tuberculosis and Lung Disease (IUATLD) recommended the use of fixed dose combination (FDC) in treatment of TB because it simplifies drug dispensing and prevents the development of drug resistance [2,3].”

According to the reviewer, this might be an historical mistake: FDC started to be recommended by WHO only in 2001 and not in 1994 when TB was declared as World-wide emergency (1993). Please check and provide reference: Bull World Health Organ. 2001;79(1):61-8. Epub 2003 Nov 5. The rationale for recommending fixed-dose combination tablets for treatment of tuberculosis. Blomberg B1, Spinaci S, Fourie B, Laing R

Reply: The document entitled “The promise and the reality of fixed-dose combinations with rifampicin. A joint statement of the International Union Against Tuberculosis and Lung Diseases and the Tuberculosis Programme of the World Health Organization. International Journal of Tuberculosis and Lung Disease, 1994, 75: 180–181” describes that the first recommendation was made by IUATLD in 1988 and that the WHO had emphasized such recommendation in 1993.

2. Lane 55 : please already mention here the most important ADR observed, related to treatment changes.

Reply: We have now added to the indicated text the most important ADR observed, which was migraine.

3. Lane 111: please explain if there was a stratification between Adverse events non requiring hospitalization (minor, mild or moderate) from Adverse events requiring hospitalization (major, severe). Comment if not and in particular try to evaluate if some ADR required hospitalization or not, if not mention it.

Reply: Only one patient reported having been hospitalized. We have added this information to the text in the Results section.

4. Lane 145: mean age for the RHEZ-FDC group was higher than to the RHZ group (40.0 ± 13 years vs. 36.4 ± 13 years; p<0.01). Please explain why, if randomly sampled, there is a significant difference of age between the two groups, in particular comment if this difference could be related to and decrease TB recent transmission rate relative to reactivation in group RHEZ versus to group RHZ.

Reply: In the period 2010 and 2014 (DATASUS-SINAN), there was an age-specific change in TB incidence. There was an increase in the median age of cases of all forms of TB and in the proportion of cases among older individuals and adults aged 30 years or older. These trends have possibly been determined by demographic factors (population growth and aging) as well as epidemiological factors (TB control programs, HIV infection). (Gaspar RS et al. J. Bras. Pneumol. 2016, vol.42, n.6, pp.416-422. SINAN - Sistema de Informação de Agravos de Notificação [Internet]. Available: http://sinan.saude.gov.br/sinan/login/login.jsf)

In addition, the drop in fertility rates that began in the mid-1960s has kept the absolute number of young people constant. This situation may have contributed to the stability in the number of TB cases in 0-39yo age groups.

Another interesting point is the improvement of tuberculosis control programs in order to reduce the annual risk of infection, which has led to a decrease in the incidence of TB observed in groups less than 30 years old. We have added this rationale in the Discussion section (line 267).

5. Lane 196-198: please comment on the reasons of the observed discrepancy between IBIT figures and Salvador figures

Reply: There are several risk factors for the development of MDR TB such as psychiatric diseases, alcoholism, drug addiction and homelessness which can predict non-adherence to treatment. Poor compliance with treatment is also an important factor in the development of acquired drug resistance. All these factors have been addressed at IBIT, with comprehensive services offered in conjunction with specialized mental health centers and psychosocial care centers as well as continuous patient support. This holistic health management performed at IBIT could be related to a discrepant results observed in Figure 3A. (Slatery, Erin, "Neglected or Non-compliant? Assessing the difficulties of tuberculosis patients in Salvador-BA, Brazil" (2014). Independent Study Project (ISP) Collection.1944. https://digitalcollections.sit.edu/isp_collection/1944). We have added this hypothesis to the Discussion section (line 318).

6. Lane 239 :”field” should replace “filed”

Reply: We have made the indicated change

7. Lane 292 : “though there was no major change in the number cases MDR-TB in Brazil at the time of implementation of the new four-drug regimen”.

I think that this point deserves more discussion, indeed, if the cure rate is not significantly different in the new regimen (point not so well discussed and difficult to estimate on Fig 2B), if there are more ADR and if MDR-TB rate does not descrease, then what is the true impact of this new regimen as mentioned two lines after ?

Reply: The discussion section was updated with the reviewer's observation (line 304)

8. The paper also mentions a Table 4 that was not found and the reference to this Table should be suppressed

Reply: We apologize for the typo, which was meant to cite Figure 3B instead of Table 4, that does not exist.

Attachment

Submitted filename: resposta.docx

Decision Letter 1

Christophe Sola

13 Dec 2019

Impact of the change in the antitubercular regimen from three to four drugs on cure and frequency of adverse reactions in tuberculosis patients from Brazil: a retrospective cohort study

PONE-D-19-25724R1

Dear Dr. Andrade,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it complies with all outstanding technical requirements.

Within one week, you will receive an e-mail containing information on the amendments required prior to publication. When all required modifications have been addressed, you will receive a formal acceptance letter and your manuscript will proceed to our production department and be scheduled for publication.

Shortly after the formal acceptance letter is sent, an invoice for payment will follow. To ensure an efficient production and billing process, please log into Editorial Manager at https://www.editorialmanager.com/pone/, click the "Update My Information" link at the top of the page, and update your user information. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org.

If your institution or institutions have a press office, please notify them about your upcoming paper to enable them to help maximize its impact. If they will be preparing press materials for this manuscript, you must inform our press team as soon as possible and no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

With kind regards,

Christophe Sola, Pharm.D., Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Acceptance letter

Christophe Sola

17 Dec 2019

PONE-D-19-25724R1

Impact of the change in the antitubercular regimen from three to four drugs on cure and frequency of adverse reactions in tuberculosis patients from Brazil: a retrospective cohort study

Dear Dr. Andrade:

I am pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department.

If your institution or institutions have a press office, please notify them about your upcoming paper at this point, to enable them to help maximize its impact. If they will be preparing press materials for this manuscript, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org.

For any other questions or concerns, please email plosone@plos.org.

Thank you for submitting your work to PLOS ONE.

With kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Pr. Christophe Sola

Academic Editor

PLOS ONE

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    S1 File. De-identified raw data extracted from the medical records.

    (XLSX)

    Attachment

    Submitted filename: resposta.docx

    Data Availability Statement

    All relevant data are within the paper and its Supporting Information files.


    Articles from PLoS ONE are provided here courtesy of PLOS

    RESOURCES