This article has been corrected: Due to errors during image assembly, the RFP-ELL1 merged image in Figure 4B is incorrect. The proper Figure 4 is shown below. The authors declare that these corrections do not change the results or conclusions of this paper.
Original article: Oncotarget. 2016; 7:29245–29254. 29245-29254. https://doi.org/10.18632/oncotarget.8588
Figure 4. Mutant EAF2K39-81-85-111R binding and co-localization with ELL1.
(A) HEK 293 cells were transfected with myc-EAF2, myc-EAF2K39-81-85-111R, or empty myc expression vector together with GFP-ELL1 or empty GFP expression vector for 36 h. The cell lysates were prepared for co-immunoprecipitation using anti-GFP antibody. The precipitates and whole cell lysates (1% input) were analyzed by immunoblotting using anti-myc and anti-GFP antibodies. GAPDH in the whole cell lysates was probed as loading control. (B) C4-2 cells were transfected with GFP, GFP-EAF2, GFP-EAF2K39-81-85-111R, RFP, and RFP-ELL1 expression vector alone or in the indicated combinations for 48 h. Subcellular localization was imaged with confocal microscopy. Image enlargement: 100×. Data shown are representative of three independent experiments.
