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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Immunity. 2019 Nov 19;51(6):1102–1118.e7. doi: 10.1016/j.immuni.2019.10.002

Figure 4. Dopamine promotes Th2 differentiation by upregulating IL-2 signaling and reducing H3K27me3 at Th2 gene loci.

Figure 4.

(A) Flow cytometry analyses of IL-2Rα (also called CD25) in Th0 cultures treated with dopamine alone and in combination with forskolin (1 μM, Fsk). Similar results were obtained in 3 independent experiments. (B) Flow cytometry analyses of CD25 and p-STAT5 in Th0+IL4 cultures of each treatment group. Similar results were obtained in 5 independent experiments. (C) Flow cytometry analyses of CD25 expression in day 2 Th0 cultures following treatment with dopamine and a STAT5 specific inhibitor, CAS285986-31-4 (20 μM). Data are representative of 3 independent experiments. (D) IL-13 production in day 2 Th0 cultures in each treatment group measured by ELISA. (E and F) The relative amount of H3K27me3 at the promoter of Il4, Il5, Il13, Il2ra and Gata3 genes in Th0 and Th0+IL-4 cultures by quantitative ChIP analyses. Bar graphs (D-F) represent mean ± SEM of three independent experiments. *p<0.05; **p<0.01 by two-tailed student’s t test. See also Figure S3.