Abstract
Structured illumination microscopy (SIM) allows for fast super-resolution imaging with low photo-toxicity, which makes it a popular fluorescent imaging technique, especially for live-cell imaging. SIM relies on both a microscope to provide precisely structured excitation light, and algorithms to merge its raw data frames into a super-resolved image in post-processing.
This talk will provide and overview of the current research into SIM image acquisition and reconstruction: The interplay of SIM instrumentation and post-processing, for both commercially available microscopesand bespoke custom solutions. For the latter, today some well documented systemsexists that have been successfullyimplemented and replicated by various optics laboratories. Examples of typical SIM imaging artifacts, how they arise and how they can be avoided during acquisition or mitigated in post-processing. Advanced SIM processing algorithms, employing iterative deconvolution approaches. These are the subject of on-going research, and are set out to solve various problems and performance issues that arise in SIM imaging. The development of high speed and low-latency SIM processing, which enables real-time super-resolution feedback in live-cell and high-throughput imaging.
In summary, both a collection of processing tools currently at our disposal, and the developments to become available within the next years, will be presented.