Figure 1.

Transcriptomic Analysis Reveals Major Differences between Human Lymphocytes Activated in HPLM^dFBS Relative to RPMI^dFBS

(A) Schematic of the comparative compositions of RPMI^dFBS, HPLM^dFBS, and HPLM^dFBS-Min. Detailed composition can be found in Table S1.

(B) Experimental outline for T cell activation in either HPLM^dFBS or RPMI^dFBS and downstream transcriptome analysis.

(C) Transcriptional differences between primary naive human mixed CD4⁺ and CD8⁺ T cells from three different donors activated in HPLM^dFBS or RPMI^dFBS as depicted in (B) were measured via RNA-sequencing. These data were used to generate PCA plots showing principal components 2 and 3 at the indicated timepoints.

(D) Heatmap showing the Log₂(fold change) in transcript abundance for genes involved in the KEGG DNA replication pathway using the RNA-sequencing data generated as described in (B).