Table 2.
Effects of genotype and age on hippocampal protein abundance evaluated by Western blot in 4- and 24-month-old IL-6-deficient (IL-6KO) and wild type control (WT) mice
| Parameter | Groups (mean ± SEM), n = 12 | p values | |||||
|---|---|---|---|---|---|---|---|
| Protein abundance | WT | IL-6KO | Young adult (4 m) | Aged (24 m) | Genotype | Age | Genotype*age interaction |
| p53 | 1.4553 ± 0.1866 | 0.8130 ± 0.0832 | 0.9447 ± 0.0473 | 1.3346 ± 0.2237 | 0.0011 | 0.0283 | 0.0099 |
| Mdm2 | 1.0357 ± 0.0542 | 0.9402 ± 0.0653 | 1.0963 ± 0.0485 | 0.8791 ± 0.0558 | > 0.05 | 0.0063 | > 0.05 |
| p21 | 0.8995 ± 0.0817 | 0.8848 ± 0.0774 | 0.9322 ± 0.0704 | 0.8521 ±0.0862 | > 0.05 | > 0.05 | > 0.05 |
| Bax | 1.0303 ± 0.0834 | 0.8740 ± 0.0798 | 0.9582 ± 0.0636 | 0.9461 ± 0.1019 | > 0.05 | > 0.05 | > 0.05 |
| Bcl-2 | 0.9230 ± 0.0433 | 0.7593 ± 0.0270 | 0.8737 ± 0.0362 | 0.8086 ± 0.0490 | > 0.05 | > 0.05 | > 0.05 |
| GFAP | 2.0029 ± 0.3326 | 2.3315 ± 0.4991 | 1.6729 ± 0.1920 | 2.6067 ± 0.5042 | > 0.05 | > 0.05 | > 0.05 |
Bold p values indicate significant influence of a given factor according to General Linear Model (GLM)