Nucleic Acids Research, 2017, 45(9): 5555–5563, https://doi.org/10.1093/nar/gkx139.
In the purification protocol, the step of tag cleavage was inadvertently omitted. The Authors wish to make the following corrections to their article.
MATERIALS AND METHODS
Protein expression and purification
Current:
… A total of 10 ml of Hepes pH 7.2, 150 mM KCl, 200 mM Imidazole was used to elute the bound protein. The elution was applied onto a 5 ml HiTrap Q HP column and subsequently washed with 4 column volumes of wash buffer…
New:
… A total of 10 ml of Hepes pH 7.2, 150 mM KCl, 200 mM Imidazole was used to elute the bound protein. The imidazole concentration in theeluate was diluted to 50 mM.Sumo-protease to a sample concentration of 0.02 mg/mL and 1mM DTT was added to the protein solution and incubated for 16 h at 4°C. The cleaved protein sample was applied onto a 5 ml HiTrap Q HP column and subsequently washed with 4 column volumes of wash buffer…
These corrections do not affect the results or conclusion of the article.
The published article has been updated.