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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Neuroscience. 2019 Nov 6;424:155–171. doi: 10.1016/j.neuroscience.2019.10.013

Table 1:

Antibodies used for single- and double-labeled immunofluorescence histochemistry.

Primary antibody Dilution Vendor Secondary antibody Dilution Vendor
Single-labeling
Rabbit anti-CCL2 1:100 Cat#:orb36895, Biorbyt Bio-Horse anti-Rabbit IgG +TSA Fluorescin 1:100 Vector
Rabbit anti-MCH 1:200 Cat#:H-070–47, Phoenix Pharmaceuticals Cy3-Donkey anti-Rabbit IgG 1:100 Jackson ImmunoResearch Laboratories
Double-labeling
Rabbit anti-CCL2 1:200 Cat#:orb36895, Biorbyt Bio-Horse anti-Rabbit IgG + TSA Fluorescein 1:100 Vector
Goat anti-pro-MCH 1:200 Cat#:sc-14509, Santa Cruz Biotechnology Cy3-Donkey anti-Goat IgG 1:100 Jackson ImmunoResearch Laboratories