Figure 3. Human pancreatic normal and cancer tissues: expression of necroptosis and apoptosis markers.

(A) Representative IF-stained tissues for DAPI (blue), CK-7⁺ tumor cells (red), and RIP-3 (green), as well as representative FACS-like co-expression scattergrams and quantitation of RIP-3 in CK-7⁺ tumor cells are blotted as mean with 95% CI as shown in Table 2. (B) Representative IF-stained tissues for DAPI (blue), CK-7⁺ tumor cells (red), and pMLKL (green), as well as representative FACS-like co-expression scattergrams and quantitation of pMLKL in CK-7⁺ tumor cells are blotted as mean with 95% CI as shown in Table 2. (C) Representative IF-stained tissues for DAPI (blue), CK-7⁺ tumor cells (red), and RIP-1 (green), as well as representative FACS-like co-expression scattergrams and quantitation of RIP-1 in CK-7⁺ tumor cells are blotted as mean with 95% CI as shown in Table 2. (D) Representative IF-stained tissues for CASPASE-3 (green), DAPI (blue) and CK-7⁺ tumor cells (red), as well as representative FACS-like co-expression scattergrams and quantitation of CASPASE-3 in CK7⁺ tumor cells are blotted as mean with 95% CI as shown in Table 2. (E) Representative images of pancreatic tumor stained for BAX (green), DAPI (blue) and CK7⁺ tumor cells (red) as well as representative FACS-like co-expression scattergrams and quantitation of BAX in CK7⁺ tumor cells are blotted as mean with 95% CI as shown in Table 2. (F) Representative images of pancreatic tumor tissue stained for cleaved CASPASE-9 (green), DAPI (blue) and CK7⁺ tumor cells (red), as well as representative FACS-like co-expression scattergrams and quantitation of CASPASE-9 in CK7⁺ tumor cells are blotted as mean with 95% CI as shown in Table 2. Human tissue scale bar = 20 μm, 20× objective. ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001, ^**** P < 0.0001.