REAGENT or RESOURCE	SOURCE	IDENTIFIER
Deposited Data
First frame of MD trajectory (.gro)	This work	10.5281/zenodo.3476169
Representative open state (.pdb)	This work	10.5281/zenodo.3476169
Trajectory of stable open state	This work	10.5281/zenodo.3476169
GlyR α1 +Gly	(Du et al., 2015)	PDB: 3JAE
X-ray structure of GlyR α3 +Gly	Huang et al., 2017b	PDB:5TIN
Cryo-EM structure of 5-HT3 receptor	Polovinkin et al., 2018	PDB:6HIN
Open structure of GLIC	Bocquet et al., 2009	PDB:3EAM
Software and Algorithms
AMBER99SB-ILDN force-field	Lindorff-Larsen et al., 2010	http://ambermd.org/AmberModels.php
Slipids force-field	Jämbeck and Lyubartsev, 2012a, Jämbeck and Lyubartsev, 2012b	http://www.fos.su.se/∼sasha/SLipids/
Zwitterionic glycine force-field	Horn, 2014	http://research.bmh.manchester.ac.uk/bryce/amber
CHAP	Klesse et al., 2019	https://www.channotation.org
gromacs	Abraham et al., 2015	http://www.gromacs.org/
MDAnalysis	Michaud-Agrawal et al., 2011	https://www.mdanalysis.org/
MODELLER 9.16	Webb and Sali, 2014	https://salilab.org/modeller/
Phenix	Adams et al., 2010	https://www.phenix-online.org/
RESMAP	Kucukelbir et al., 2014	http://resmap.sourceforge.net
UCSF Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
VMD 1.9	Humphrey et al., 1996	http://www.ks.uiuc.edu/Research/vmd/