FIG 3.

SHIV.CH505.375H.dCT replication kinetics prior to and following ATI in adult RMs. (A) Schematic representation of SHIV.CH505.375H.dCT infection (0 to 12 weeks), ART (12 to 24 weeks), and ATI (24 to 32 weeks) in adult RMs. Blood samples were collected at weekly intervals throughout the study, and peripheral lymph nodes (LNs) were collected at 12 wpi and after 8 weeks of ART (20 wpi). I.V., intravenous. (B) The kinetics of plasma SHIV RNA over 32 weeks were measured by qRT-PCR. (C) Peripheral lymph nodes from macaques on ART (20 wpi) were collected, and the level of naive, memory, and Tfh CD4⁺ T cell-associated SHIV DNA was estimated by qPCR. (D and E) The amounts of cell-associated SHIV DNA (CA-SHIV DNA) (D) and cell-associated SHIV RNA (CA-SHIV RNA) (E) from CD4⁺ T cells of peripheral blood before ART (6 wpi for DNA and 12 wpi for RNA) and on ART (18 wpi) were monitored by ddPCR. The sensitivity of the ddPCR assay was detection of 1 SHIV gag copy in 10,000 CD4⁺ T cells. Therefore, only those animals that had ≥10,000 CD4⁺ T cells at a particular time point were included in the analysis. Each symbol represents an individual animal. Yellow and gray boxes represent the duration of ART (weeks 12 to 24) and the duration of ATI (weeks 24 to 32), respectively. Medians are indicated as horizontal lines on the dot plots.