Fig. 2.

GSIS is higher in female but not male offspring that were previously exposed to maternal obesity. (a, b) Beta cell (a) and alpha cell (b) mass in offspring from control and obese dams at 8 weeks of age. (c) Insulin secretion stimulated by low glucose (2.8 mmol/l glucose; LG), high glucose (16.7 mmol/l glucose; HG) and 10 mmol/l leucine/glutamine. (d) Islet insulin content. Experiments were performed on islets isolated from 8-week-old male and female offspring of control and obese dams. Data were analysed by two-way ANOVA followed by a simple main effect analysis when a significant interaction was observed. There was a significant interaction between maternal diet and offspring sex for data relating to basal (p < 0.05), glucose-stimulated (p < 0.01) and leucine/glutamine-stimulated (p < 0.05) insulin secretion, indicating a sex-specific effect on the outcome measured. *p < 0.05 and **p < 0.01 as shown. (a, b) Males, n = 4 mice/group; females, n = 6 mice/group; (c) LG: males, control: n = 8, obese: n = 6 mice; females, control: n = 6, obese: n = 7 mice; HG: males, control: n = 9, obese: n = 7 mice; females, control: n = 7, obese: n = 8 mice; Leu/Gln: males, control: n = 8, obese: n = 5 mice; females, control: n = 6, obese: n = 8 mice; (d) males and females, n = 5 mice/group. ‘n’ represents the number of mice from separate litters. All data are mean ± SEM