TABLE 1.
Flagellation phenotype | % presenting phenotype (mean ± SD) in strain: |
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WT | ΔflhG | ΔfliF | ΔfliG | ΔfliM | ΔfliY | ΔfliN | ΔfliH | ΔfliI | ΔfliM ΔfliY | ΔfliM ΔfliN | ΔfliY ΔfliN | |
All cellsb | ||||||||||||
Normalc | 89.4 ± 0.8 | 51.8 ± 5.7 | 0 ± 0 | 0 ± 0 | 32.6 ± 0.7 | 40.1 ± 0.4 | 68.6 ± 4.4 | 12.3 ± 1.1 | 30.1 ± 4.1 | 31.0 ± 2.6 | 34.8 ± 5.3 | 29.0 ± 4.7 |
Amphitrichous | 72.4 ± 2.5 | 8.8 ± 0.7 | 2.9 ± 0.7 | 4.7 ± 0.2 | 33.2 ± 3.2 | 0.3 ± 0.4 | 1.3 ± 1.9 | 1.3 ± 0.7 | 2.6 ± 1.1 | 0.8 ± 1.1 | ||
One at one pole | 17.0 ± 3.3 | 43.0 ± 5.0 | 29.8 ± 1.3 | 35.4 ± 0.2 | 35.4 ± 1.2 | 11.9 ± 0.6 | 28.8 ± 6.0 | 29.7 ± 3.3 | 32.1 ± 6.4 | 28.3 ± 3.6 | ||
Aflagellated | 8.5 ± 1.4 | 8.4 ± 2.0 | 100 ± 0 | 100 ± 0 | 62.8 ± 0.1 | 58.0 ± 0.5 | 21.7 ± 1.9 | 87.7 ± 1.1 | 69.5 ± 3.5 | 65.2 ± 1.6 | 63.8 ± 4.5 | 70.2 ± 4.7 |
Hyperflagellatedd | 2.1 ± 0.6 | 39.8 ± 3.7 | 0 ± 0 | 0 ± 0 | 4.6 ± 0.6 | 1.9 ± 0.9 | 9.7 ± 2.5 | 0 ± 0 | 0.4 ± 0.6 | 3.8 ± 0.9 | 1.5 ± 0.9 | 0.7 ± 0 |
Flagellated cells onlye | ||||||||||||
Normalc | 97.7 ± 0.6 | 56.5 ± 5.0 | 87.7 ± 1.6 | 95.6 ± 2.1 | 87.5 ± 3.5 | 100 ± 0 | 98.4 ± 2.2 | 89.7 ± 3.2 | 95.7 ± 2.8 | 97.5 ± 0.3 | ||
Hyperflagellatedd | 2.3 ± 0.6 | 43.5 ± 5.0 | 12.3 ± 1.6 | 4.4 ± 2.1 | 12.5 ± 3.5 | 0 ± 0 | 1.6 ± 2.2 | 10.9 ± 3.2 | 4.3 ± 2.8 | 2.5 ± 0.3 |
Two experiments were performed in which >100 individual cells were analyzed for flagellation by transmission electron microscopy.
Includes data from all cells analyzed regardless of the flagellation phenotype.
The normal flagellation phenotype includes cells that are amphitrichous (producing a single flagellum at each pole) and cells producing a single flagellum at one pole (“one at one pole”).
Cells producing 2 or more flagella at least at one pole.
Only includes the population of flagellated cells, divided into normally flagellated cells (amphitrichous and one flagellum at one pole) and hyperflagellated cells.