Figure 2:

A-485rs inhibitory effects on p300 acetyltransferase activity in the presence or absence of I-CBP112 in a purified system. (A) Dose-dependent inhibition of full-length p300 catalyzed acetylation of 10 μM histone H3 by 0–10 μM A-485rs in the presence or absence of 20 μM I-CBP112 monitored by Western blot (anti-H3K18Ac). (B) Quantification of replicate results (n = 2) in (A) were fit to a standard dose response curve to determine the respective IC₅₀’s. Error bars represent standard error of replicates; IC₅₀ comparison p = 0.54.