Figure 5:

Effects of A-485rs and I-CBP112 on mRNA levels and p300 occupancy of KLK3 and c-myc promoter and enhancer regions in LNCaP cells. mRNA levels of KLK3 (A) and c-myc (B) were measured by qRT-PCR after 24 h of treatment with increasing concentrations of A-485rs with or without 20 μM I-CBP112. LNCaP cells were treated with the indicated ligands for 24 h followed by ChlP-PCR of p300-bound KLK3 enhancer (C) and promoter (D). Representative plot of biological duplicates each done with n = 3. * p < 0.05, ** p < 0.005, *** p < 0.0005