Fill-in mix (add in this order) | Per well |
---|---|
10x NEBuffer 2 | 0.5 μl |
20 mM dATP (Thermo Scientific, FERR0181) | 1 μl |
Klenow (exo-) (5 U/ μl, Thermo Scientific, EP0422) | 0.3 μl |
water | 3.2 μl |
Fill-in mix (add in this order) | Per well |
---|---|
10x NEBuffer 2 | 0.5 μl |
20 mM dATP (Thermo Scientific, FERR0181) | 1 μl |
Klenow (exo-) (5 U/ μl, Thermo Scientific, EP0422) | 0.3 μl |
water | 3.2 μl |