Fig. 1.

Growth kinetics of three virus serotypes in suspension-adapted HEK293 T-rex cells.

Each column of graphs represents a different serotype (as indicated by column captions), and each row a different measured parameter (as indicated by row captions: virus particle titer, assayed by qPCR; infectious unit titer; and viable cell density respectively). Each parameter was measured at multiple timepoints after infection. Blue lines indicate MOI of 3; red lines indicate MOI of 10. Solid lines and filled symbols indicate cell-associated virus [assays using cell pellets]; dashed lines and open symbols, where present, indicate virus in supernatant. Points and error bars indicate the median and range of two independent flasks within an experiment; absence of a visible error bar indicates close inter-replicate agreement. For each flask the plotted results are the mean of triplicate qPCR measurements and duplicate infectivity titers. Results are representative of two independent experiments for ChAdOx2 and ChAd63. In the case of ChAdOx1, a second experiment performed using a different transgene insert (a different viral glycoprotein) also gave similar results (data not shown).