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The study design for speeding up the sample preparation. Two different washing procedures (“ON”: washing the multicellular tumor spheroid once with PBS on the 96-well plate vs. “OFF” washing three-times in an Eppendorf-tube after transfer from cultivation well) and two alternative extractions (boiling ethanol vs. cold methanol extraction) were tested on large multicellular tumor spheroids (744 ± 15 µm), which in combination resulted in four sample groups.
