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. 2019 Dec 7;11(12):1134. doi: 10.3390/v11121134

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lysogen isolation and testing. (a) Liquid phage release from WheeHeim lysogens. Serially diluted stock Streptomyces phage WheeHeim (row 1), streak purified S. scabiei (WheeHeim) lysogen supernatant (row 2), and stock Streptomyces phage Forthebois (row 3) spotted on NA⁺ overlayed with S. scabiei RL-34 after 48 h at 30 °C. (b) Superinfection immunity test of S. scabiei (Forthebois) lysogen. Serially diluted crude lysate stocks of Streptomyces phage Scap1 (row 1) Forthebois (row 2), WheeHeim (row 3), and S. scabiei (Forthebois) lysogen supernatant (row 4) spotted on NA⁺ overlayed with S. scabiei (Forthebois) lysogen after 48 h at 30 °C.