Figure 6.

Effect of DTT on the inhibitory effect of Calebin A, and/or curcumin in TNF-β-induced p65-NF-κB binding to DNA. Extracted nuclei of TNF-β-treated CRC cell lines were co-treated with Calebin A (CA), and/or curcumin in the absence or presence of DTT and nuclear extracts then immunoblotted for p65-NF-κB activation. The results are demonstrated from at least three independent assays and the housekeeping protein β-actin served as an internal loading control. Densitometric evaluation was performed for phospho-p65 for all CRC cell lines. * p < 0.05, ** p < 0.01.