Figure 2.

Calpain inhibitor cross-reacts with viral proteases. A549 cell homogenate was incubated with purified viral proteases 2A and 3C in the presence or absence of 200 µM calpain inhibitor (ci) or 250 µM elastatinal (ela) for 18 h at 22 °C. Western blot analysis was done by resolving the samples in 4–20% SDS-PAGE gels and transferring to PVDF membrane. The blots were immunolabeled with PABP and G3BP1 antibodies, and the total protein amount was assessed using a stain-free method. Arrow indicates 3C protease induced cleavage fragment of G3BP1.