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. 2019 Dec 10;11(12):3023. doi: 10.3390/nu11123023

Figure 3.

Figure 3

Ellagic acid inhibits expression of COX1 and COX2, which are involved in acidity-enhanced invasion. (A,B) AGS and SNU601 cells maintained in acidic medium were treated with 100 μM sulindac or 30 μM SC58635 for 24 h. Then, the number of invaded cells was counted. (C–J) AGS (C–F) and SNU601 (G–J) cells maintained in acidic medium were treated with sulindac (C, E,G,I) or SC58635 (D,F,H,J) for 24 h. The mRNA expression of the genes encoding MMP7 (C,D,G,H) and MMP9 (E,F,I,J) was subsequently analyzed by real-time PCR. (K,L) AGS (K) and SNU601 (L) cells maintained in normal or acidic medium were further incubated for 24 h with or without ellagic acid. Then, total protein extracts were analyzed by immunoblotting with antibodies against COX1, COX2, and NHE1 (left), and densitometry calculations for immunoblotting data are shown (right). * p < 0.05 vs. no treated control at pH 7.4; # p < 0.05 vs. no ellagic acid at pH 6.5.