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. 2020 Jan 3;51:102604. doi: 10.1016/j.ebiom.2019.102604

Fig. 1.

Fig 1

The protein levels of PRAS40 in HCC tissue and its correlation to the survival rate of HCC patients. a–d. Analyses of 22 pairs of primary HCC and peri‑cancer tissue samples in Cohort 1. HE and IHC staining of PRAS40 in HCC and peri‑cancer tissue (a). Degrees indicating the intensity of PRAS40 staining in representative HCC tissue (b). H-scores multiplied by the intensity and extent of PRAS40 staining in HCC and peri‑cancer tissue (c). The correlation of PRAS40 protein level to the survival rate of HCC patients (d). e-f. H-scores of PRAS40 staining (e) and p-PRAS40 staining (f) in 44 pairs of primary HCC and peri‑cancer tissue samples in Cohort 2. g-h. The correlation of PRAS40 protein level (g) and phosphorylation level (h) to the survival rate of 50 HCC patients in Cohort 3. i-j. RNA-seq results of AKT1S1 mRNA in HCC and normal liver tissue samples in public TCGA dataset. The relative AKT1S1 mRNA levels were compared in 371 cases of HCC and 50 cases of normal liver tissue (i). The correlation of AKT1S1 mRNA level to the survival rate of 365 HCC patients (j). k-l. PRAS40 protein levels in the livers of DEN-injected Akt1s1+/+ mice were evaluated by Western blotting (k). The quantitative results were shown in l. Scale bars, 100μm. N, non-tumor; T, tumor. Bars, SD. **, P<0.01 from Student's t-test.