Fig. 4.

The effects of PRAS40 depletion on HCC xenograft formation in nude mice. HepG2 cells were introduced with control, PRAS40 shRNA (shPRAS40) or PRAS40 shRNA together with shRNA-resistant PRAS40 expression vector (shPRAS40+PRAS40). a-d. The cells of each group were inoculated into the two posterior flanks of the mice, n = 6. Tumor volumes were recorded every 2–3 days (a). On day 19, tumors were dissected and obtained (b-c). Pictures were taken at the sacrificed time (b). Tumor weights were measured (c). Bars, SD. *, P<0.05; **, P<0.01 (Student's t-test). d. Cell lysates were applied to Western blotting. e. Quantitative results of the six samples from each group. Bars, SD. *, P<0.05; **, P<0.01 (Student's t-test).