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. 2019 Dec 4;8(12):1570. doi: 10.3390/cells8121570

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RNA binding proteins interaction. (A) REMSA experiments performed using 3DQA101 (Lane 1) and 3DQA105 (Lane 9) riboprobes. Lanes 2 and 8 show the digestion of riboprobes with T1 RNase. The binding of DQA101 with M14 extract is in Lane 3 and with B-LCL#5 extract in Lane 6. The binding of 3DQA105 with M14 extract is in Lane 9 and with B-LCL#5 extract in Lane 12. The interaction of 5 and 1 µg of rTTP with 3DQA101 is shown in Lanes 4 and 5 and with 3DQA105 in Lanes 10 and 11. (B) Western blot analysis of biotin pull-down assay carried by using 3DQA101, 3DQA105, 3DQB102, and 3DQB105 riboprobes. The antibodies used for the immunoblot were anti-NF90, anti-TTP, and anti-EBP1. Molecular weights are as indicated.