Analysis of gene expression on hMSCs after EGF treatments and EGFR signaling gene expression investigation on Nh-Ost. qRT-PCR was performed after 24 h of specific transfection on hMSCs. EGFR and ERK3 expression levels were evaluated on hMSCs after miR-33a-3p over-expression or inhibition or after miR-33a-5p over-expression or inhibition (A) qRT-PCR was performed on Nh-Ost, EGF, EGFR, ERK-2, and ERK-3 (B) Quantitative RT-PCR data are expressed as fold of change (FOI) in gene expression (2−ΔΔCt) that occurred in hMSCs after treatments vs. scramble groups and in Nh-Ost vs. hMSCs cells. Student t test: * p < 0.05, ** p < 0.005, *** p < 0.0005 between experimental group.