Figure 3.

A three‐band PCR strategy confirms the presence of mice with ectopic germline recombination. (a) Schematic diagram depicting the expected PCR products from primers flanking both loxP sites. (b) Foxp3‐YFP‐Cre^– conventional T (Tconv) cells and Foxp3‐YFP‐Cre⁺ regulatory T (Treg) cells were sorted from the spleens of Foxp3^YFP‐Cre mice, Foxp3^YFP‐CreInsr^fl/wt mice, and Foxp3^YFP‐CreInsr^fl/fl mice. PCR was performed with cell lysates, and PCR products were resolved on a 1% agarose gel. Each lane represents one mouse. (c) Punnett square showing the expected offspring genotype ratio of a test cross of Foxp3^YFP‐CreInsr^wt/rcb parents (left). Table compares expected and actual test‐cross offspring ratios (right). (b) Data from two independent experiments. (c) n = 21 in the F₅ generation.