Table 1.
Genotype ratios of all mice in the second breeding cohort from Figure 6
| F1 | Male (n = 18) | Female | Total (n = 18) |
|---|---|---|---|
| Foxp3YFP‐Cre/yInsrwt/fl | 9 (50%) | No data | 9 (50%) |
| Foxp3YFP‐Cre/yInsrwt/fl‐r | 9 (50%) | No data | 9 (50%) |
| F2 | Male (n = 66) | Female (n = 53) | Total (n = 119) |
|---|---|---|---|
| Foxp3YFP‐CreInsrwt/fl | 8 (12%) | 8 (15%) | 16 (13%) |
| Foxp3YFP‐CreInsrwt/fl‐r | 23 (35%) | 18 (34%) | 41 (34%) |
| Foxp3YFP‐CreInsrwt/wt | 29 (44%) | 21 (40%) | 50 (42%) |
| Foxp3YFP‐CreInsrwt/rcb | 6 (9%) | 6 (11%) | 12 (10%) |
| F3 | Male (n = 81) | Female (n = 86) | Total (n = 167) |
|---|---|---|---|
| Foxp3YFP‐CreInsrfl/fl | 4 (5%) | 4 (5%) | 8 (5%) |
| Foxp3YFP‐CreInsrfl/fl‐r | 5 (6%) | 12 (14%) | 17 (10%) |
| Foxp3YFP‐CreInsrfl/rcb | 11 (14%) | 4 (5%) | 15 (8%) |
| Foxp3YFP‐CreInsrwt/fl | 4 (5%) | 7 (8%) | 11 (7%) |
| Foxp3YFP‐CreInsrwt/fl‐r | 34 (42%) | 39 (45%) | 73 (43%) |
| Foxp3YFP‐CreInsrwt/wt | 11 (14%) | 11 (13%) | 22 (13%) |
| Foxp3YFP‐CreInsrwt/rcb | 12 (15%) | 9 (10%) | 21 (12%) |
| F4 (knockout) | Male (n = 88) | Female (n = 75) | Total (n = 163) |
|---|---|---|---|
| Foxp3YFP‐CreInsrfl/fl | 43 (49%) | 36 (48%) | 79 (48%) |
| Foxp3YFP‐CreInsrfl/fl‐r | 45 (51%) | 39 (52%) | 84 (52%) |
Using a qPCR genotyping strategy, mice with > 40% ear tissue gDNA containing ectopic recombination were considered to have a Insrrcb allele; ectopic recombination within 5–40% of ear tissue gDNA was denoted as Insrfl‐r; and < 5% ectopic recombination was considered normal (Insrfl)