KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat Monoclonal anti-mouse CD16/32 (Fc receptor blocker) | BioLegend | Cat#101302 |
| Rat monoclonal anti-B220-eF450 | eBioscience | Cat#48–0452-82 |
| Rat monoclonal anti-human/mouse B220-PE | eBioscience | Cat#12–0452-83 |
| Rat monoclonal anti-mouse/human B220-FITC | BioLegend | Cat#103206 |
| Rat monoclonal anti-mouse/human B220-APC-Cy7 | BioLegend | Cat#103223 |
| Armenian hamster monoclonal anti-mouse CD3e-FITC | eBioscience | Cat#11–0031-82 |
| Rat monoclonal anti-mouse CD11b-FITC | eBioscience | Cat#11–0112-82 |
| Rat monoclonal anti-mouse Ly-6C-Alexa 488 | eBioscience | Cat#53–5932-82 |
| Armenian hamster monoclonal anti-mouse CD11c-FITC | eBioscience | Cat#11–0114-82 |
| Rat monoclonal anti-mouse CD49b-FITC | eBioscience | Cat#11–5971-82 |
| Mouse monoclonal anti-mouse FAS-APC | BioLegend | Cat#152604 |
| Rat monoclonal anti-mouse CD38-PE | BioLegend | Cat#102708 |
| Rat monoclonal anti-mouse CD138-PE | BioLegend | Cat#142504 |
| Rat monoclonal anti-mouse CD138-PerCP Cy5.5 | BioLegend | Cat#142509 |
| Rat monoclonal anti-mouse CD138-APC | BioLegend | Cat#142506 |
| Rat monoclonal anti-mouse CD138-Biotin | BioLegend | Cat#142511 |
| Rat monoclonal anti-mouse Ly-6G-FITC | BioLegend | Cat#127605 |
| Rat monoclonal anti-mouse Blimp1-Alexa 647 | BioLegend | Cat#150003 |
| Rat monoclonal anti-mouse cRel- PE | eBioscience | Cat#12–6111-80 |
| Rat monoclonal anti-mouse cRel-eF660 | eBioscience | Cat#50–6111-80 |
| Mouse monoclonal anti-Rela-PE | Cell Signaling | Cat#9460 |
| Rabbit polyclonal anti-Rela | SantaCruz | Cat#sc-372 |
| Rabbit polyclonal anti-cRel | SantaCruz | Cat#sc-71 |
| Rat monoclonal anti-Blimp1 | SantaCruz | Cat#sc-47732 |
| Goat polyclonal anti-IRF4 | SantaCruz | Cat#sc-6059 |
| Rabbit polyclonal anti-Bach2 | Abcam | Cat#ab83364 |
| Goat polyclonal anti-Actin | SantaCruz | Cat#sc-1615 |
| Rabbit polyclonal Anti-Histone H3 (acetyl K9) | Abcam | Cat#ab4441 |
| Rabbit isotype IgG | SantaCruz | Cat#sc-2027 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| fixable viability dye eF506 | Affymetrix Bioscience | Cat#65–0866-18 |
| 7 aminoacitomycine D (7AAD) | BioLegend | Cat#420404 |
| Critical Commercial Assays | ||
| Ig isotyping ELISA kit | eBioscience | Cat#88–50630-88 |
| Dead Cell Apoptosis Kit | ThermoFisher Scientific | Cat# V13241 |
| CellTrace™ Far Red Cell Proliferation Kit | ThermoFisher Scientific | Cat#C34564 |
| Fix and Perm cell permeabilization kit | ThermoFisher Scientific | Cat#GAS003 |
| Public data set | Nature Immunology, 2015,16(6):663–73 | GSE60927 |
| Public data set | Nature Immunology, 2016,17(3), 323–330 | GSE70981 |
| Public data set | Nature Immunology, 2016, 17(3), 331–343 | GSE71698 |
| Experimental Models: Cell Lines | ||
| HEK 293 | ATCC | CRL-1573 |
| BCL1 | ATCC | CRL-1669 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6 | The Jackson Laboratory | JAX: 006494 |
| Mouse: C57BL/6: rel-/- | Kontgen et al., 1995, Genes & development 9, 1965–1977 | N/A |
| Oligonucleotides | ||
| pLX-cRel, Bach2 KO guide RNA (5’-CACCGGAACTTTCGTCCCCCTGCGC-3’, 5’-CACCGTGCTGCAGGGACGGGCACAA-3’) | This paper | N/A |
| Blimp1 KO guide RNA (5’-CACCGGAATCCAGCTCACTCTGCCC-3’, 5’-CACCGACCTGGCTGCCTGTCAGAAC-3’) | This paper | N/A |
| Blimp1 binding site disruption guide RNA (5’-CACCGTACTAGAGCATCTGAAAGCC-3’) | This paper | N/A |
| Blimp1 PCR primer: ACTTAAAGCCTTTTTGTGCTTCT and CAGTTTCCTTTACAGCAGGAGTT | This paper | N/A |
| Software and Algorithms | ||
| Graphpad Prism | GraphPad Software | |
| Mathworks MATLAB | MathWorks, Inc | R2014a |
| Other | ||
| All Computational Modeling Code | This paper | http://www.signalingsystems.ucla.edu/models-and-code/ |