Figure 4.
ZDHHC17 is Important for Brain Glioma Development. (A, B) Migratory human neural stem cells (hNSCs) transfected with indicated lentiviruses in Transwell assays. Cells were stained with crystal violet for counting. Scale bar, 500 µm. Data represent the means ± SD from three separate experiments (*p < 0.05; ***p < 0.001, unpaired t-test). (C) Western blot of markers Oct 4 (stem cell self-renewal), Ki67 (proliferative), and GFAP (glial) and MAP2 (neuronal) in TERT/CA-KRAS/DN-p53/ZDHHC17 hNSCs compared to hNSCs. (D, E) Immunofluorescence (D) and positive percentage (E) of GFAP and MAP2 in TERT/CA-KRAS/DN-p53/ZDHHC17 compared to hNSCs. Scale bar, 50 μm. Data represent the means ± SD from three separate experiments (**p < 0.01; ***p < 0.001, unpaired t-test). (F, G) C57BL/6 mice were subcutaneously injected with 1 × 105 hNSCs transfected with indicated lentiviruses. Tumor volumes were measured every three days. Each point represents the mean volume ± SD of five tumors (**p < 0.01; ***p < 0.001). After 3 weeks, nude mice were sacrificed; the dissected tumors are shown.