Fig. 3.

Representative OR-PAM label-free triple-view VYS of an embryo at E12.5. (a), (b), and (c) refer to coronal, transverse, and sagittal planes of VYS microvascular, respectively; a representative region in the white dashed boxes to illuminate the dealing process of image in Fig. 3(a). (d) feature map after the Hessian matrix treated; (e) extracted microvessels binarized image overlaid with the vessel centerlines image. (f) and (g) statistical analysis of vessel density and vessel diameter in VYS of embryo at three different development stages, respectively. Bar: 1 mm in (a) and (c), 500 μm in (b).