Skip to main content
. 2019 Oct 23;9(1):1676615. doi: 10.1080/2162402X.2019.1676615

Figure 4.

Figure 4.

Design and expansion of cCD20-BB-ζ canine CAR T cells.

(a) Diagram of cCD20-BB-ζ construct. (b) CAR surface expression on live CD5+ cells before and 7 days after sequential stimulations with irradiated CLBL-1 cells at 1:1. (c) CAR T cell counts at baseline and 7 days after stimulations 1 and 3 and 9 days after stimulation 2. (d) Specific lysis of distinctly labeled GL1-GFP-CD20 cells using GL1-GFP cells as controls after 4 hour co-culture with 5:1, 1:1 or 1:5 T cell: target cells 19 days after transduction, mean and SD of triplicate wells displayed. (e) Canine interferon γ secretion quantified using ELISA following 24-hour co-culture of 1:1 T cell: target cells 20 days after transduction, mean and SD of triplicate wells displayed.