Figure 5.

Use of single PCR to re-verify the co-carriage of tet(X3) and tet(X5) in two isolates of CMG11-2 and FS35-1 PCR results of single gene verified that the two strains (CMG11-2 and FS35-1) are consistently positive in tet(X3) [and tet(X5)]-specific molecular detection, whereas negative in tet(X1) [or tet(X2)/tet(X4)]-specific PCR assays. The expected size of PCR products is 685 bp for tet(X3), and 265 bp for tet(X5) (Table 1). PCR products were separated with electrophoresis of 2% agarose gel. Designations: bp, base pair; M, DNA marker (Trans 2K Plus II); the symbol “–”, negative control; the mixture of tet(X) [X1 to X5] PCR products, positive control.