Figure 4.

Neutralization antibodies titer against MERS-CoV in mice sera vaccinated with inactivated chimeric bivalent 5 + 3 and MERS-CoV viruses. Plaque reduction neutralization test 50 (PRNT₅₀) was used for measuring antibodies titers in mice sera in Vero-E6 cells. The experimental data represented by mean of three replicates ± SEM (seven mice/group). Statistical analysis was performed using repeated measures ANOVA, followed by Bonferroni post hoc test. The significant differences are indicated (* = p < 0.05, ** = p < 0.01, *** = p < 0.001 and nonsignificant = ns).