Figure 5.

VPA1701 directly bound to the promoter of VPA0264. (A) qRT-PCR analysis of the transcription level of lateral flagellar genes (VPA0264, VPA1537, VPA1538, VPA1539, VPA1540, and VPA1548) cultured in BHI agar plates at 30 °C. The data are presented as mean ± SD (n = 3); *: p < 0.01; **: p < 0.05; ***: p < 0.001. (B) EMSAs were performed with purified VPA1701 protein, and the promoter region of the VPA0264 was analyzed. 15 ng of FAM-labeled probes were added to the EMSA reaction and mixed with non-specific competitor DNA (poly (dI:dC)).