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. 2019 Nov 9;8(4):227. doi: 10.3390/pathogens8040227

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Development of BBD over an infection time course from 24–72 HAI with P. tolaasii strain Pt011W inoculum suspension on mushroom caps of three Pleurotus strains (P0, P282, and PPU) in a controlled environment. (a–c) Blotch symptom severity with increasing levels of inoculum dosage at 24, 48, and 72 HAI, respectively. (d) Symptom progression on the three strains using the optimum inoculum dosage. Disease severity (DS) rating was based on a 0–5 scale according to the extent of tissue discoloration and nature, where 0 = no symptom; 0.5 = very pale lesion difficult to distinguish from the cap; 1 = level yellowish lesion; 2 = level brown lesions; 3 = slightly sunken lesion; 4 = extensive brown sunken lesion; 5 = extensive dark sunken lesions occasionally with sticky cytosolic material [28]. Data are mean disease severity scores of two trials (at 18 °C and 21 °C), each with six bags treated (three fruiting caps inoculated/bag)/strain. Bars are the standard errors of the means.